Pathobiologeical Studies Of Infectious Bursal Disease Virus (Ibdv) In Experimentally Infected Birds (Record no. 3478)

000 -LEADER
fixed length control field 02518nam a2200193Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151006153428.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s2014xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 1770,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Maria Ali
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Muti-ur-Rehman Khan
245 ## - TITLE STATEMENT
Title Pathobiologeical Studies Of Infectious Bursal Disease Virus (Ibdv) In Experimentally Infected Birds
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2014
502 ## - DISSERTATION NOTE
Dissertation note Poultry industry is rapidly growing under the shelter of livestock, with the contribution of 11.4% of value addition in GDP. However infectious and noninfectious diseases are major threat to the growth of poultry industry. Among the infectious diseases, infectious bursal disease (IBD) is one of the major malady which causes great economic losses to poultry in terms of both morbidity and mortality. IBD virus was first identified in 1962 in gumboro, since then the disease spread to all parts of world. Until 1980s IBD virus was efficiently controlled by use of vaccine however with the emergence of variant strains of virus it was become difficult to control the disease. Studies on molecular structure of IBDV revealed that it has a hyper variable region in VP2 gene. Mutations in this hyper variable region lead to emergence of different strain of virus and hence causing vaccine failure.
In Pakistan there is an outbreak of disease each year despite of vaccination. So there was a need to characterize field isolate and vaccine isolate of IBDV to find any mutation in field isolate of virus. The current study was designed by keeping above factors in mind. Purpose of present study was to compare local field and vaccine isolate of virus. RNA was isolated by TRIzol method from both vaccine isolate and bursa collected from field. RNA was converted to cDNA by using oligoDT. In next step VP2 gene was amplified by using a set of primers P1 and P2.
cDNA was digested by 2 enzymes BstN1 and MboI. Results of RFLP showed that there was a difference in genetic sequence of both strains. Howeverthe pattern of disease produced by both of the strains was same.
Chicks were given experimental infection at age of 2 week and slaughtered after 3 days post infection. Gross and histopathological lesions were observed and compared to each other and also with negative control. Statistical analysis was done by using one way ANOVA followed by Duncan's multiple range test.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Pathology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Aftab
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Ishtiaq Ahmed
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-06-01 1770,T 1770,T Thesis


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