Identification Of Variations In The Coding Region Of Myostatin Gene In Thalli And Pakkarakul Sheep Breeds Of The Punjab (Record no. 6104)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03444nam a22002177a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151008134056.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150907b2015 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2288-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Armughan Ahmed Wadood (2006-VA-38) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Afzal Ali |
| 245 ## - TITLE STATEMENT | |
| Title | Identification Of Variations In The Coding Region Of Myostatin Gene In Thalli And Pakkarakul Sheep Breeds Of The Punjab |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2015. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 49p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | In a developing country like Pakistan, livestock sector has a significant importance. Meat is an important food component in human diet and its source varies from different organisms like cattle, buffalo, camel, deer, sheep, goat, rabbit and chicken etc. Small ruminants especially sheep, has a unique role in meat production, due to its feeding and adaptive behavior. The myostatin (MSTN) gene is known as a candidate gene for development and growth of livestock because it plays its major role in muscle growth. Selection is an important tool to improve the meat production and thus enhance the economic conditions of livestock farmers. For this purpose genetically characterization of meat type animals, especially the indigenous sheep breeds is not common. The variations in the myostatin gene have been extensively studied and reviewed in small and large ruminants in the world but it has been scanty studied in sheep breeds of Pakistan. Thalli and Pak-Karakul are well known sheep breeds present in Punjab, Pakistan. This study aims to characterize the genetic variation in the myostatin gene in Thalli and Pak-Karakul sheep breeds. In this research study, twenty adult animals of two different sheep breeds (Thalli and Pak-Karakul) were selected from Small Ruminants Research and development Centre, Rakh Khairewala, District Layyah, Pakistan. Five mL blood sample was collected from each animal in a 15mL falcon tube containing anticoagulant. Primers were designed by using Primerfox online software. Primers were optimized using specific protocol and PCR was performed. DNA was extracted using modified inorganic method of Sambrook et al. (1989). PCR was carried out using all primers and later sent for sequencing to 1stBase Laboratories, Singapore. Molecular analysis was done using CodonCode Aligner and MEGA6 softwares. Ten samples of each breed were sequenced to detect polymorphism in both Thalli and Pak-Karakul sheep populations. Sequencing revealed G↓T transition at 3995 position in Summary 44 genome (accession number, DQ530260.1) in Thalli and similar transition in Pak-Karakul at the same position. In Thalli sheep 50 % of experimental animals were heterozygous, higher level of heterozygosity makes it a potential candidate for higher growth rates. On the other hand the Pak-Karakul sheep is medium weight breed and frequency of mutant allele was 0.2 or 20%. The observed heterozygous individuals were also with higher live body weights. The gene frequency shows that this breed has medium potential for its body growth. The information‟s so generated and further association studies in both breeds will be helpful in devising breeding plans for increasing mutton production at national level. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Animal Breeding and Genetics |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Livestock Production |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Khalid Javed |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mr. Muhammad Saadullah |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-09-07 | 2288-T | 2288-T | Thesis |