Pathobiological Studies On Bovine Ephemeral Fever Infected Cattle In District Swabi (Record no. 6117)

000 -LEADER
fixed length control field 03097nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151008133944.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150908b2015 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2284-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Sahibzada Waheed Abdullah (2013-VA-560)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Muti Ur Rehman Khan
245 ## - TITLE STATEMENT
Title Pathobiological Studies On Bovine Ephemeral Fever Infected Cattle In District Swabi
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2015.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 36p.;
502 ## - DISSERTATION NOTE
Dissertation note Among the non-contagious diseases Bovine ephemeral fever is important disease of cattle the course of the disease is usually three days due to which it is called “three days sickness”. This is transfer to other cattle through insects Culicoides (biting midges a group that include many kinds of flies) and mosquitoes. Bovine ephemeral fever virus (BEFV) has been collected from Culicoides coarctatus, Culicoides brevitarsis, and Anopheles bancroftii (Walker et al. 2012). Cattle and buffaloes are the main species affected from Bovine ephemeral fever (BEF) which gives huge economic losses to the dairy sector. The etiologic agent, Bovine ephemeral fever virus belong to Rhabdoviridae family, enveloped (negative sense) ssRNA virus. It generally recur in Australia, Asia, and Africa also in the Middle East (Walker 2005).
The theme of the present study was detection of BEF virus through Reverse Transcriptase-Polymerase Chain Reaction from the cattle suspected for bovine ephemeral fever virus on the basis of clinical signs. Hematological profile and serum calcium level were checked in the confirmed positive samples for BEFV.
A total of 50 blood samples were collected from the suspected animals in aseptic condition using a sterilized disposable syringe and were preserved in vacutainers (Anticoagulant added n = 50, without anticoagulant n = 50). The 10 blood samples were collected from healthy animals in vacutainers (Anticoagulant added n = 10, without anticoagulant n = 10).
Buffy coat were separated from blood samples and from this the RT-PCR was performed and successfully diagnosed the BEFV infected cattle. Hematology and serum calcium were performed for both confirm positive and healthy animals.
Summary
31
The result showed that BEF virus was diagnosed with the help of RT-PCR in samples suspected for BEFV infection, and there was no virus detected in samples taken from healthy animals. Comparison of hematology between BEFV infected cattle and healthy animals were performed there was no changes in the RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, and MID (it include monocyte, eosinophils, and basophils) except Neutrophils, which number was increases and lymphocytes which was decreased in BEFV infection, while in healthy animals there was no change in the whole hematology. Serum calcium was also determined there was decrease in serum calcium level of BEFV infected cattle, while in the healthy animal samples there was no change in the serum calcium level.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Pathology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Asim Aslam
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Ali Ahmad Shiekh
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-09-08 2284-T 2284-T Thesis


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