Detection Of Brucella Species From Aborted Bovine Fetuses Using Amos Pcr And Immunohistochemistry (Record no. 6371)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02766nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151020123735.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151020b xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2320-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Muhammad Naveed Anvar (2008-VA-310) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Raheela Akhtar |
| 245 ## - TITLE STATEMENT | |
| Title | Detection Of Brucella Species From Aborted Bovine Fetuses Using Amos Pcr And Immunohistochemistry |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 39p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | The economic importance and public health concern of bovine brucellosis enlist it in the world top priority disease to be eliminated by WHO (World Health Organization). Bovine brucellosis is caused by three Brucella species including B. abortus, B. melitensis and B. suis. Although the literature explains that the incidence of B. abortus is higher in bovines but still we need to know the prevalence of other species particularly B. melitensis in Pakistan due to its zoonotic aspect which makes it more important for study. Unfortunately in Pakistan the status of B. abortus and B. melitensis in bovines is unknown. It is need of hour to determine the exact prevalence of B. abortus and B. melitensis in bovines for disease eradication in animals, to control its transmission in humans and to determine the reasons behind vaccine failure in bovines. B. abortus and B. melitensis could be presents in aborted bovine samples. AMOS PCR can be better tool than immunohistochemistry for the detection of B. abortus and B. melitensis from aborted bovine samples. (Hypothesis) A total of 60 tissue samples (lung, liver and stomach) from aborted bovine fetuses were collected from farms with history of abortion and suspected brucellosis in and around Lahore district. The samples were subjected to AMOS PCR and immunohistochemistry for detection of B. abortus and B. melitensis. Brucella abortus and Brucella mellitensis species specific primer were used to get the desired base pair. The genomic region of B. abortus IS711was amplified at 498bp. From present study it can be concluded that brucellosis is present in cows and buffaloes at district Lahore and it is more in cattle as compared to buffaloes. Therefore an immediate actions and policies are required to be implemented for the preventing spread of the disease to the other animals and human. For the diagnosis of Brucella species AMOS PCR and immunohistochemistry were used and the results showed that Brucella abortus were more as compared to other species in aborted bovine tissues. The results also showed that the sensitivity and specificity of AMOS PCR is more than immunohistochemistry. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Pathology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Gulbeeena Saleem |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Jawad Nazir |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-10-20 | 2320-T | 2320-T | Thesis |