Antibacterial And Cytotoxic Evaluation Of Different Extracts Of Zingiber Officinale Rhizome Against Common Poultry Pathogens (Record no. 6380)
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| 000 -LEADER | |
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| fixed length control field | 03371nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151020134917.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151020b2015 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2324-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Ghalia Qayyum (2013-VA-779) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Aqeel Javeed |
| 245 ## - TITLE STATEMENT | |
| Title | Antibacterial And Cytotoxic Evaluation Of Different Extracts Of Zingiber Officinale Rhizome Against Common Poultry Pathogens |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 121 p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Antimicrobial compounds having plant origin inhibit bacteria through different mechanisms and can be used for the treatment of infections against resistant microbes. Majority of antibacterial drugs in clinical use are derived from natural origin. Hence, the present study is designed for antibacterial and cytotoxic evaluation of different extracts of Zingiber officinale rhizome against common poultry pathogens. The four sequential i.e. hexane, chloroform, ethanol and aqueous extracts of Zingiber officinale were prepared by soxhlet extraction. Antibacterial activity of these extracts was determined by agar well diffusion method against Staphylococcus aureus, Clostridium perfringens type A, Escherichia coli, Salmonella enterica and Haemophilus paragallinarum. Zone of inhibitions were determined by well diffusion method. MICs of plant extracts were determined by micro broth dilution method. Cytotoxic activity was evaluated by applying MTT assay on Vero cell lines The zone of inhibitions showed by hexane, chloroform, ethanol and aqueous extracts of Zingiber officinale against Staphylococcus. aureus were 12.33mm, 13.67mm, 16.33mm and 14mm; against Clostridium perfringens type A were 12mm, 16.33mm, 14mm and 8.33mm; against Escherichia coli were 14.33mm, 13.33mm, 14.33mm and 12mm; against Salmonella enterica were 17mm, 17.33mm and 12mm; against Haemophillus paragallinarum were 12.67mm, 13mm and 14mm respectively. Hexane extract showed no zone of inhibition against Salmonella enterica and aqueous extract was ineffective against Haemophillus paragallinarum. MICs values of hexane, chloroform, ethanol and aqueous extracts of Zingiber officinale rhizome against Staphylococcus. aureus were 2500µg/ml, 625µg/ml, 2500µg/ml and 2083.33µg/ml; against Clostridium perfringens type A were 2500µg/ml, 312.5µg/ml, 1250µg/ml and 5000µg/ml; against Escherichia coli were 5000µg/ml, 1250µg/ml, 5000µg/ml and 5000µg/ml; against Salmonella enterica were 312.5µg/ml, 5000µg/ml, 5000µg/ml; against Haemophillus paragallinarum were 2500µg/ml, 1458.33µg/ml and 2500µg/ml respectively. MIC was not performed against hexane extract of Salmonella enterica and aqueous extract of Haemophillus paragallinarum as no zone of inhibition observed against them. Hexane extract of Zingiber officinale rhizome was cytotoxic at concentration ≥ 750µg/ml, chloroform extract at concentration ≥ 1500µg/ml and aqueous extract at concentration ≥5000µg/ml. Ethanol extract at concentration ranging from 1500µg/ml to 2.92µg/ml was not cytotoxic to cell. The indigenous plant Zingiber officinale have antibacterial activity against common poultry pathogens and helpful to develop new drug from plant origin. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Pharmacology and Toxicology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Qamar Niaz |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Aftab Ahmad Anjum |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-10-20 | 2324-T | 2324-T | Thesis | |
| UVAS Library | UVAS Library | 2015-10-20 | 2324-T | Thesis |