Comparison Of Antifungal Activity Of Human Salivary Histatin Between Diabetic And Nondiabetic Individuals (Record no. 6499)

000 -LEADER
fixed length control field 02702nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151109084956.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 151109b2015 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2341-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Farid-Ul-Haq (2013-VA-555)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Prof. Dr. Tahir Yaqub
245 ## - TITLE STATEMENT
Title Comparison Of Antifungal Activity Of Human Salivary Histatin Between Diabetic And Nondiabetic Individuals
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2015
300 ## - PHYSICAL DESCRIPTION
Number of Pages 44p.;
502 ## - DISSERTATION NOTE
Dissertation note Histatins are antimicrobial proteins found in human saliva. These proteins have also been
observed to have the ability to aid in wound healing in various organisms. The genes HTN1 and
HTN3 have been studied to govern these proteins. Histatin proteins have a vast array of
antimicrobial properties. While a fungus, Candida albicans or C. albicans is a part of the human
normal gut flora, it is a threat to people who have a compromised immune system. An
overgrowth of the fungi belonging to the Candida family leads to candidiasis in humans, and oral
candidiasis has been reported to a large extent namely in diabetic patients. The antifungal
activity of histatin proteins laid the basis of the current research work.
In this study, the antifungal activity of saliva from a total of 64 healthy and diabetic
human samples against Candida albicans has been evaluated. The samples of both healthy and
diabetic human samples belong from different age ranges: 15-25, 25-35, 35-45 and 45-55 years
in order to change in antifungal activity with respect to age of an individual. Antifungal activity
was observed through both agar well and agar disk diffusion methods, with agar disk diffusion
methods showing positive results. According to the outcomes of this study at least 120μL of
healthy saliva sample is required to create a zone of inhibition. Saliva from diabetic individuals
showed no antifungal results.
This occurrence led to the next part of this study involving amplification of HTN3 gene.
The nucleotide sequences of both healthy and diabetic individuals were compared together and
showed that the absence of antifungal activity in diabetic individuals might have reasons other
than a genetic one, according to this study. The results observed from the present study indicate
that healthy human saliva possesses antifungal activity against Candida albicans. In accordance
Summary
39
to these results, the naturally occurring antimicrobial activity of histatin proteins present in
human saliva can have immense use in the field of medicine.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Molecular Biology and Biotechnology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Ali Raza Awan
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Muhammad Tayyab
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-11-09 2341-T 2341-T Thesis


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