Comparison Of Antifungal Activity Of Human Salivary Histatin Between Diabetic And Nondiabetic Individuals (Record no. 6499)
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fixed length control field | 02702nam a22002057a 4500 |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20151109084956.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 151109b2015 xxu||||| |||| 00| 0 eng d |
041 ## - LANGUAGE CODE | |
Language code of text/sound track or separate title | eng |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
Classification number | 2341-T |
100 ## - MAIN ENTRY--AUTHOR NAME | |
Personal name | Farid-Ul-Haq (2013-VA-555) |
110 ## - MAIN ENTRY--CORPORATE NAME | |
Location of meeting | Prof. Dr. Tahir Yaqub |
245 ## - TITLE STATEMENT | |
Title | Comparison Of Antifungal Activity Of Human Salivary Histatin Between Diabetic And Nondiabetic Individuals |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Year of publication | 2015 |
300 ## - PHYSICAL DESCRIPTION | |
Number of Pages | 44p.; |
502 ## - DISSERTATION NOTE | |
Dissertation note | Histatins are antimicrobial proteins found in human saliva. These proteins have also been observed to have the ability to aid in wound healing in various organisms. The genes HTN1 and HTN3 have been studied to govern these proteins. Histatin proteins have a vast array of antimicrobial properties. While a fungus, Candida albicans or C. albicans is a part of the human normal gut flora, it is a threat to people who have a compromised immune system. An overgrowth of the fungi belonging to the Candida family leads to candidiasis in humans, and oral candidiasis has been reported to a large extent namely in diabetic patients. The antifungal activity of histatin proteins laid the basis of the current research work. In this study, the antifungal activity of saliva from a total of 64 healthy and diabetic human samples against Candida albicans has been evaluated. The samples of both healthy and diabetic human samples belong from different age ranges: 15-25, 25-35, 35-45 and 45-55 years in order to change in antifungal activity with respect to age of an individual. Antifungal activity was observed through both agar well and agar disk diffusion methods, with agar disk diffusion methods showing positive results. According to the outcomes of this study at least 120μL of healthy saliva sample is required to create a zone of inhibition. Saliva from diabetic individuals showed no antifungal results. This occurrence led to the next part of this study involving amplification of HTN3 gene. The nucleotide sequences of both healthy and diabetic individuals were compared together and showed that the absence of antifungal activity in diabetic individuals might have reasons other than a genetic one, according to this study. The results observed from the present study indicate that healthy human saliva possesses antifungal activity against Candida albicans. In accordance Summary 39 to these results, the naturally occurring antimicrobial activity of histatin proteins present in human saliva can have immense use in the field of medicine. |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical Term | Department of Molecular Biology and Biotechnology |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Personal name | Dr. Ali Raza Awan |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Personal name | Dr. Muhammad Tayyab |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Thesis |
Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-11-09 | 2341-T | 2341-T | Thesis |