Observation Of Antibacterialactivity Of Human Salivary Histatin Against Staphylococcus Aureus (Record no. 6502)

000 -LEADER
fixed length control field 02854nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151109092715.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 151109b2015 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2342-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Rizwan Irshad (2013-VA-10)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Professor Dr. Tahir Yaqub
245 ## - TITLE STATEMENT
Title Observation Of Antibacterialactivity Of Human Salivary Histatin Against Staphylococcus Aureus
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2015
300 ## - PHYSICAL DESCRIPTION
Number of Pages 44p.;
502 ## - DISSERTATION NOTE
Dissertation note The saliva contains numbers of proteins which act as antimicrobial agents and these peptides called as antimicrobial peptides. The saliva contains numerous antimicrobial peptides the main salivary peptide present in saliva is Histatin protein rich in Histidine amino acid. Histatin is only antimicrobial peptides present in primates like monkey chimpanzees and human. It’s also contain anti-fungal as well as anti-bacterial effect against staphylococcus aureus. In this study showed the effect of human salivary Histatin against staphylococcus aureus. Salivary Histatin may show antibacterial activity against Staphylococcus aureus with its efficiency having link to age, gender and diabetic status. Total sixty-four saliva sample will be collected on the basis of gender, age and presence of diabetic status. The antibacterial activity of saliva was observed against Staphylococcus aureus by disc-diffusion method. DNA was extracted and HTN1 gene was amplified using specific primer. This study was helpful in demonstrating antimicrobial ability of Histatin proteins present in human saliva.
It also provide insight with regards to age, sex and/or immunocompromising ailment (in this case, diabetes) having an effect on the ability of these proteins, thus, opening new doors when it comes to combating fungal infections in both human and animal subjects.
The HTN1 gene sequenced and BLAST results proof that variation in Histatin anti-bacterial property in diabetic patients and was not due to mutation in the nucleotide sequences of the decreases in salivary Histatin was due to other reason not due to mutation in these individuals.
The age bases study HTN1 gene BLAST results was found 99% similarity with the other age groups.
Summary
40
The statistical analysis of healthy people with age and zone of inhibition was found ANOVA P<0.000. The increases of age will decreases the salivary Histatin anti-bacterial properties. The optimum antibacterial activity was measured 2cm in diameter.
The present results indicated that healthy human saliva possess antibacterial ability against Staphylococcus aureus. The results indicated that salivary Histatin can be novel tool as antimicrobial peptides of future medical field.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Molecular Biology and Biotechnology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Muhammad Wasim
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Nisar Ahmad
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-11-09 2342-T 2342-T Thesis


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