Assessment Of Microbial Load, Protease Activity And Aflatoxin M1 In Raw And Uht Milk Procured From Local Markets Of Lahore (Record no. 6517)
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| fixed length control field | 03101nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151109110824.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151109b2015 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2354-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sadaf Almas (2007-VA-250) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Imran Altaf |
| 245 ## - TITLE STATEMENT | |
| Title | Assessment Of Microbial Load, Protease Activity And Aflatoxin M1 In Raw And Uht Milk Procured From Local Markets Of Lahore |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 115p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Pakistan is among the largest milk producing countries. The requirement of milk is increasing day by day. It has rapidly increasing demand and competition in national and international markets. Milk consumers in Pakistan often face low-quality, lack of hygiene and absence of cold chains as primary contributors to this low quality. Milk and dairy products also become contaminated during manufacturing and packaging processes. The consumption of low quality milk may cause milk borne diseases. Not only the bacteria but the presence of their enzymes also can cause deterioration of the milk quality. The heat stable enzymes can cause the spoilage of commercial UHT products without presence of any viable count. Aflatoxin (M1) is metabolite of AFB1 in milk. It causes chronic diseases and immunosuppression in children. It is found carcinogenic and cytotoxic in nature. In this project microbial load, free amino acid estimation to predict any protease activity and Aflatoxin M1 were studied in both UHT and Raw milk samples (n=15) procured from local markets of Lahore. Three UHT brands A, B and C were purchased. The UHT milk was studied for microbial growth and protease activity at purchase and at expiry of the products. The microbial load was evaluated by testing of milk for Total viable count, Coliforms, Yeast and Molds, Anaerobic Clostridia and Bacillus cereus in both raw and UHT milk. Protease activity was estimated by assessing the free amino acid by using ninhydrin assay while the Aflatoxin M1 was detected through High performance liquid chromatography. SUMMARY 80 CONCLUSION: It was found that the locally available raw milk quality was poor for consumption and dairy processing for safe and stable milk products. UHT milk quality was found better with low microbial load. Protease activity with reference to free amino acid was detected in raw milk which is indication of the poor milk storage conditions, cold chain maintenance and unavailability of fresh milk. Protease activity was also found in UHT milk and an increase in free amino acid which could be due to heat stable proteases active during shelf life of the milk brands. Aflatoxin M1 was found in majority of raw milk sample which showed the poor animal feed storage and monitoring system. Aflatoxin M1 was also found in some samples of UHT brands with high concentration which depicted that AFM1 was heat stable and it retained in the commercial UHT products as well. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Ali Ahmad Sheikh |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Muhammad Nasir |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-11-09 | 2354-T | 2354-T | Thesis |