Seroprevalence Of Brucellosis In Pregnant And Aborted Women Of Rural And Urban Areas In Three Selected Districts Of Punjab Pakistan (Record no. 6600)

000 -LEADER
fixed length control field 04119nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151130094733.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 151130b2015 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2359-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Shakeela Anjum (2006-VA-172)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Iahtasham khan
245 ## - TITLE STATEMENT
Title Seroprevalence Of Brucellosis In Pregnant And Aborted Women Of Rural And Urban Areas In Three Selected Districts Of Punjab Pakistan
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2015.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 56p.;
502 ## - DISSERTATION NOTE
Dissertation note Brucellosis is an important zoonotic disease of animals and human. According to OIE
(Office International des Epizooties), it is the second most important zoonotic disease in the
world after rabies Brucellosis is more common in countries with Poorly standardized animal
and public health programme. Human Brucellosis caused by B. abortus, B. abortus are small,
non-motile, aerobic, facultative intracellular, Gram-negative cocobacilli.
The symptoms of human brucellosis are undulant fever, headache, weakness, body
pain, and sometimes endocarditis, orchitis, or arthritis may develop. Prolonged use of
combination of antibiotics and human vaccine is main control measure strategies for human
brucellosis. This disease is under investigated and hence people are ignorant of this insidious
problem. Serological surveillance is a good and cheap tool to diagnose this problem. There is
also need to know that which diagnostic antigen (Pakistani or French) is more sensitive and
specific from disease eradication and control point of view. Unfortunately annual incidence
of brucellosis in whole Pakistan is unknown but they are expected above 2 per 100,000
people. The hypothesis of present study was that brucellosis is considered to be endemic in
Pakistan but there is no data available about seroprevalence of brucellosis in pregnant,
aborted, rural and urban women with different risk factors in Pakistan.
In present study sero-screening of total 199 serum samples of pregnant and women
presenting with abortions from district Jhang, district Chinoit and district Faisalabad. Test
was performed on 199 samples which showed 40 (20%) positives by RBPT (Pakistan) and 42
(21%) positives by RBPT (France) (Table 4.4). While the indirect modified ELISA showing
5 (2.5%) positive samples. All sera tested positives (5) with modified i-ELIA were also tested
positives with another specific and highly expensive ELISA (Verion Serion) at OIE reference
Summary
41
Laboratory for brucellosis, Germany. Prevalence data was analyzed by chi square test
using SPSS version 20 Software (Apache License, USA) to find out correlation between risk
factors and brucellosis prevalence.
Risk factors such as consumption of raw milk, contact with animals, and symptoms
were significant. In our opinion, indirect modified ELISA is more sensitive than RBPT
(Pakistan) and RBPT (French). RBPT, (Pakistan) can be used for primary screening of
brucellosis cases because of cross reactivity present in RBPT antigen and confirmation must
be made with a more specific and sensitive serological test, such as B. abortus-specific
indirect modified ELISA. The results of present study showed that RBPT, Pakistan antigen
showed almost same sensitivity and specificity as that of RBPT (Pourquier, France) antigen.
Indirect modified ELISA is more specific as compared to RBPT (Pakistan) and RBPT
(French). It is highly suggestive to combine serodiagnostics test with molecular detection
including PCR and Real time PCR to increase the detection rate of brucellosis. Human
ELISA kit is extremely expensive that cannot be afforded in developing countries like
Pakistan to screen the human. PCR technique is highly reliable and less time consuming. It is
highly suggestive to conduct the study on human brucellosis on other districts of south
Punjab as it is believed that brucellosis is endemic due to lack of awareness and vaccination
in these areas.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Epidemiology and Public Health
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Abdul shakoor
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Muhammad Younus
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-11-30 2359-T 2359-T Thesis


Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:rehana.kousar@uvas.edu.pk Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.