Optimization Of Loop-Mediated Isothermal Amplification (Lamp) For The Molecular Diagnosis Of Feline Babesiosis (Record no. 7136)

000 -LEADER
fixed length control field 02157nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20160118093727.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 160118b2015 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2374-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Muhammad Awais Salim (2012-va-606)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Muhammad Lateef
245 ## - TITLE STATEMENT
Title Optimization Of Loop-Mediated Isothermal Amplification (Lamp) For The Molecular Diagnosis Of Feline Babesiosis
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2015.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 27p.;
502 ## - DISSERTATION NOTE
Dissertation note Babesia is a worldwide tick borne hemoparasite causing Babesiosis, an important disease affecting a number of animals and attracting the researcher’s attention due to its zoonotic potential.Babesiosis in cats often presents as a chronic and low grade disease, however most common symptoms include anaemia, lethargy, weakness and rarely icterus and fever.
Blood samples were collected from 100 domestic cats at Pet Center, UVAS, Lahore,from their ear tips and cephalic/saphenous vein. The blood will be immediately transferred to EDTA coated vacutainers. Stained thin blood smears were observed for intra-erythrocytic bodies and 45 samples were selected after screening. Blood in EDTA were tested for PCR (already optimized) in the Molecular Parasitology laboratory at UVAS, Lahore, to screen for B. felis. ExtractedDNA of confirmed B. felis samples were further processed forLoop-Mediated Isothermal Amplification (LAMP).
LAMP primers weredesigned recognizing four sections of the B. felis gene. LAMP reactions of 25µL were standardized at 60°C temperature for 1 hour time using DNA extracted from blood samples of cats found positive on PCR.
Briefly, the concentration of FIP and BIP were varied from 0.8µM to 2.4µM, Mg2+from 2mM to 4mM, betaine from 0.2 to 0.8M and dNTPs from 1mM to 4mM.The LAMP reaction was optimized at the final concentration of 0.2µM F3 and B3, 2.0µM of each of the FIP & BIP, 2mM for each dNTPs, 0.8M betaine, 1X reaction buffer, 1µl bst polymerase and 2µl DNA templates at 60°C.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Parasitologhy
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Azhar Maqbool
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Muhammad Nauman Zahid
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2016-01-18 2374-T 2374-T Thesis


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