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| fixed length control field |
02216nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20160307102410.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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160307b2015 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
2389-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
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| Personal name |
Muhammad Salman (2008-VA-135) |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
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| Location of meeting |
Prof. Dr. Khalid Saeed |
| 245 ## - TITLE STATEMENT |
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| Title |
Molecular Diagnosis Of Anaplasmosis In Buffaloes |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Year of publication |
2015. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Number of Pages |
46p.; |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Bovine Anaplasmosis is a tick-borne haemo-rickettsail disease, caused by Anaplasma species transmitted mechanically by flies, biologically by ticks and blood contaminant fomites. It is an economically important tick-borne disease of buffalo in tropical and sub-tropical areas of the world. In current study, we developed and optimized PCR first for detecting Anaplasma at genus level in buffaloes. One hundred (100) blood samples were collected from buffaloes around the Lahore region. The stained thin blood films were examined microscopically and 37% blood samples were found positive for intra-erythrocytic bodies which were then selected for DNA extraction. The DNA was extracted using commercially available kit for eventual use in optimization of PCR for diagnosis of bovine Anaplasmosis. The primers were designed targeting 16S rRNA gene of Anaplasma. For the detection, the PCR product was run in 2% agarose gel stained with ethidium bromide and thirty seven samples showed the amplification band at 179bp. The selected samples were sent for ABI sequencing to Singapore for the accurate detection of the Anaplasma species. The sequencing results were blasted with database of Genbank and we observed homology with Anaplasma phagocytophilum. We found 37% prevalence of Anaplasmosis in buffaloes through PCR. However more studies are required to confirm the species of Anaplasma infecting buffaloes (Bobalus bobalis) by designing species specific primers. Furthermore, additional studies are needed to establish the epidemiology of Anaplasmosis by using molecular tools in different geographical areas of the country for their better control.
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Department of Parasitology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dr. Muhammad Imran Rashid |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dr. Wasim Shehzad |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Thesis |
