Detection Of Amantadine Resistant Variants Among Avian Influenza Viruses Subtype H9n2 Isolated In Pakistan (Record no. 8665)

000 -LEADER
fixed length control field 02256nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20160628102850.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 160628b2016 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2457-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Sabir Subhan (2009-VA-32)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Prof. Dr. Tahir Yaqub
245 ## - TITLE STATEMENT
Title Detection Of Amantadine Resistant Variants Among Avian Influenza Viruses Subtype H9n2 Isolated In Pakistan
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2016.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 42p.;
502 ## - DISSERTATION NOTE
Dissertation note Avian influenza A virus subtype H9N2 is prevalent in poultry industry of Pakistan. Amantadine is an antiviral drug which is being used as prophylactic measure to control this disease despite the occurrence of resistance against this drug. There is need to monitor the resistance of amantadine in Flu viruses. In this study, we collected 100 samples of broilers birds showing mild to severe respiratory signs. Samples were collected from different locations of Punjab, Pakistan. After initial identification via Hemagglutination test, the molecular identification and confirmation of subtype H9N2 was done by multiplex PCR. To rule out the co-infection of NDV, PCR of NDV was also done. The samples which were pure H9N2 were further processed for the screening of amantadine susceptibility. To do this, titration of viruses was done on MDCK cells in the presence and absence of amantadine at the concentration of 2 ug /ml. The results of TCID5O were compared in the presence and absence of amantadine for each isolate and isolates showing difference of 2 log 10 TCID50/0.1 ml were declared resistant to amantadine as described by Masuda et al. 2000. The results of this study revealed that the viruses circulating in the poultry industry if Pakistan are resistant to this drug as we found that out 10 isolates 4 were resistant to this drug. So, there is need to monitor the usage of this drug in poultry as human cases of H9N2 viruses have been reported and virus was of avian origin. Monitoring is necessary because amantadine is recommended in flu pandemics and this virus possesses the pandemic potential and can cross the species barrier.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Microbiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Muhammad Zubair Shabbir
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Yasin Tipu
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2016-06-28 2457-T 2457-T Thesis


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