||Glycerin has gained attraction being a low cost and energy rich compound that can partially replace the major ingredients including corn and some other energy rich stuffs. It may work as an alternative energy source without any detrimental effect on production performance, egg quality and hatching traits in Japanese quails. This study evaluated subsequent effect of glycerin on productive performance, egg quality and hatching traits in Japanese quail. A total of 200 birds were arranged according to completely randomized design into 5 treatment groups having 5 replicates of 8 birds each (6 female + 2 Males). Treatment consisted 5 levels of glycerin i.e., 2.5, 5, 7.5 and 10 % and control group. Birds were fed with different levels of glycerin during rearing period of six weeks and their subsequent effect on productive performance, egg quality and hatching traits were observed. Data were collected regarding productive performance for 16 weeks, however, egg quality and hatching traits were recorded during pre-peak (at 4th week), peak (at 12th week) and post peak (at 16th week) phase. No significant effect of glycerin on subsequent productive performance, egg quality and hatching traits were observed throughout the experimental period. Regarding productive performance, initial and final body weight, feed intake, hen housed and hen day production%, egg weight, egg mass, FCR / dozen egg and / kg egg mass did not show any major significant difference in all three phases. However, glycerin had significant effect on subsequent egg weight during peak and post peak phase. In egg quality, no significant effect of glycerin were observed on subsequent egg shell thickness, shell weight and yolk index in all three phases, whereas, albumen height and Haugh Unit score during pre-peak and peak phase were effected significantly. In terms of hatching traits, hatchability, fertility, dead in shell and dead germ% did not show any significant difference in all phases. However, clear egg % showed significant difference during peak phase of production.