Molecular Phylogeny And Diversity Analysis Of Bovidae (Boselaphus Tragocamelus, Antilope Cervicapra) And Cervidae (Axis Axis, Axis Porcinus) In Pakistan (Record no. 9265)
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| fixed length control field | 02375nam a22002297a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20160919131522.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160919b2016 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2560-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Ghulam Abbas (2011-VA-748) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Asif Nadeem |
| 245 ## - TITLE STATEMENT | |
| Title | Molecular Phylogeny And Diversity Analysis Of Bovidae (Boselaphus Tragocamelus, Antilope Cervicapra) And Cervidae (Axis Axis, Axis Porcinus) In Pakistan |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2016. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 134p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Many species of mammals have declined within the past two centuries due to human caused disturbances and the unsustainable use of natural resources. Molecular methods have an important role in phylogeny and diversity analysis. The present study was designed for diversity analysis of Boselaphus tragocamelus & Antelope cervicapra (Bovidae) and Axis axis & Axis porcinus (Cervidae) family in Pakistan. A total of 25 samples from each of the four species were collected from different parks, zoos and natural habitats. DNA was extracted, PCR primers were designed and cytochrome-b, cytochrome-c gene and d-loop regions were amplified by PCR. PCR products were sequenced bi-directionally by Big DyeTM Terminator. Bioinformatics tools, Blast 2 sequences, Clustal-W, MEGA-6, Bioconductor in “R” were applied for analysis. The clustering of the samples indicates that each species contains less within-population genetic variability. Same pattern was observed when sequence of three genes was combined and MDS plot was constructed. Phylogenetic analysis of the gene sequences revealed that each species comprised a clade that is clearly distinct from the clade comprised of other species of deer selected for this study. Finding of this study indicated that these species of deer have significant genetic variations among-species that differentiate them from each other. This is the first report from our region. The information of selected species of deer is prerequisite for designing effective strategy in future conservation practices. However further genomic investigations should be carried out at larger scale. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Molecular Biology and Biotechnology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | IBBT |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Phd thesis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Mansoor Ellahi Babar |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Muhammad Tayyab |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2016-09-19 | 2560-T | 2560-T | Thesis |