Method Development And Validation For The Estimation Of Sofosbuvir By Reversed-Phase High Performance Liquid Chromatography (Record no. 9476)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02345nam a22002177a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20161024144003.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161024b2016 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2593-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Mohsin Ali (2008-VA-586) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Shaista Qamar |
| 245 ## - TITLE STATEMENT | |
| Title | Method Development And Validation For The Estimation Of Sofosbuvir By Reversed-Phase High Performance Liquid Chromatography |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2016. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 53p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Aim of the present work was to develop a UV/Visiblespectroscopic and isocratic reversed phase high performance liquidchromatography (RP-HPLC) method for estimation of Sofosbuvir, a novel drug molecule employed for the treatment of hepatitis C virus infection.Both of the methods were validated as per ICH guidelines.For UV/Vis method the solvent used was analytical grade methanol and maximum absorbance was obtained at wavelength 260 nm. The responsewas linear in range of 10 – 50 µg/ml with a regression coefficient of 0.999. For this method limit of detection (LOD) value and limit of quantification value were 0.25µg/ml and 0.84µg/ml respectively. The reversed phase chromatographic separation was achieved on a C18 column (5 µm,150 x 4.6 mm) in isocratic mode using mobile phase containing 0.1 % Phosphoric acid : Acetonitrile (60:40, % v/v) at ambient temperature (25°C). The pH of mobile phase was finally adjusted to 3.0 by using triethylamine. The flow rate was 1.0 ml/min and the detection was carried out at 260 nm and a sharp peak was obtained at retention time of 4.40 ± 0.01 min. The linear regression analysis data revealed that a good relationship occur between response and concentration in the range of 5 – 80 µg/ml. The regression coefficient was found out to be 0.999. The detection (LOD) and quantification (LOQ) limits as computed from peak to noise ratio was 0.50 µg/ml and 1.51 µg/ml. It was proved from statistical analysis of results that data obtained by spectroscopic and chromatographic methods are highly accurate precise and reproducible and hence can beused for quality control and for further stability testing of Sofosbuvir. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Pharmaceutical Chemistry |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Pharmaceutical Sciences |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Nabeel Shahid |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Abdul Muqeet Khan |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2016-10-24 | 2593-T | 2593-T | Thesis |