Evaluation Of Inhibition Activity Of Indigenous Lactobacilli Spp. On Ammonia Emitting Bacteria (Record no. 9477)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02569nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20161024144627.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161024b2016 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2594-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Fatima Sajjad (2010-VA-312) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Muhammad Nawaz |
| 245 ## - TITLE STATEMENT | |
| Title | Evaluation Of Inhibition Activity Of Indigenous Lactobacilli Spp. On Ammonia Emitting Bacteria |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2016. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 61p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Poultry is the 2nd largest industry in Pakistan which is growing at an amazing rate of more than 10% from last few years. It provides jobs for more than 1.5 Million people. Although, it is growing at excellent pace, it still faces many problems. One of the important problems in poultry farming is the production of ammonia by urease producing microbes. Ammonia is health hazard for both poultry and human. Urease producing bacteria which are the major problems in poultry are Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae and Staphylococcus aureus in poultry droppings. Probiotics such as Lactobacilli can inhibit the urease producing bacteria in poultry intestine as well as environment thus mitigate the ammonia emission. Present study was designed to isolate Lactobacilli from indigenous poultry, screen them for antimicrobial activity against urease producing microbes and determine their effect on growth of Proteus mirabilis during co-culture experiments. A total of 71 Lactobacilli isolated were recovered from 20 samples of droppings (10) and caeca (10) of back-yard poultry on MRS agar plates. Twenty seven (27) isolates demonstrated antimicrobial activity against ammonia emitting bacteria by agar spot and well diffusion assays. Seven isolates (FSL19, FSL25, FSL39, FSL45, FSL51, FSL63 and FSL71), having better antimicrobial activity, were selected for co-culturing with Proteus mirabilis in nutrient broth. FSL25, FSL45, and FSL51 showed more than 2 log10 reduction of Proteus mirabilis in co-culture experiments. FSL25, FSL45, and FSL51 were identified as Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus salivarius, respectively by amplifying and sequencing their partial 16S rDNA. It is concluded that Lactobacillus plantarum FSL25, Lactobacillus fermentum FSL45 and Lactobacillus salivarius FSL51 may be used to mitigate ammonia emitting bacteria in poultry environment after further investigations. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Masood Rabbani |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Sehrish Firyal |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2016-10-24 | 2594-T | 2594-T | Thesis |