Conversion Of Pretreated Rice Husk Hydrolysates Into Ethanol By Saccharomyces Cerevisiae
By: Jehanzaib (2014-VA-08)
| Dr. Rahat Naseer.
Contributor(s): Miss Faiza Masood | Prof. Dr. Saima Naveed.
Material type: 
BookPublisher: 2016Description: 56p.Subject(s): Biochemistry | IBBTDDC classification: 2644-T Dissertation note: Biodegradable wastes wereresidues of commercial crops.Agricultural wastes such as rice husk was utilized for biofuel production such as ethanol by breaking downinto its components using chemicals or enzymes. This was achievedthrough anaerobic digestion, distillation and fermentation for use as a resource of heat and fuel (bioethanol).Bioethanol meansan alternative to petrol which is produced from plants such as sugar cane or maize and rice husks.Agricultural wastes can be utilized for the biofuel, rice husk often pose.Acid and alkali pretreatment was enhanced the yield of ethanol from rice husk.Rice husk wastreated with alkali and acids to produce glucose. Glucose can be estimated byusing glucose oxidase method (GOD). Glucose was then converted into ethanol using Saccharomyces cerevisiae. Ethanol production was estimated by using spectrophotometer.The purposed project wasfocusing on bioethanol from the rice husk residues in which rice husk was pre-treated for the hydrolysis to produced maximum yield of sugar by selection of best pretreated method. And then sugars were converted into bioethanol by fermentation. Alkaline solutionswere remove lignin, while the acidic solutionswere remove hemicellulose. It was clearly showed in the graph.4.1 and 4.2 that best concentration of alkali 4% for the maximum yield of sugar was 50.4 mg/dl from the maximum 15g substrate of rice husk was used at 70˚C for 3hrs while in case of 4% acid treatment was also best for the maximum 42.6mg/dl sugar yield was obtainedat 70˚C for 3hrs.
According to statistical analysis and graphs 4.3 and 4.4 showed that maximum yieldi.e, 68.7 ml ethanolwas produced from the 15g rice husk when treated with 4%alkali,maximum temperature 35˚C with PH 6.0 and after 3days.While in case of 4%acid treatmentmaximum 63.7 ml ethanol was producedwhen maximum 15g rice husk was used at temperature 35˚C andPH with 6.0.after 3days.
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Biodegradable wastes wereresidues of commercial crops.Agricultural wastes such as rice husk was utilized for biofuel production such as ethanol by breaking downinto its components using chemicals or enzymes. This was achievedthrough anaerobic digestion, distillation and fermentation for use as a resource of heat and fuel (bioethanol).Bioethanol meansan alternative to petrol which is produced from plants such as sugar cane or maize and rice husks.Agricultural wastes can be utilized for the biofuel, rice husk often pose.Acid and alkali pretreatment was enhanced the yield of ethanol from rice husk.Rice husk wastreated with alkali and acids to produce glucose. Glucose can be estimated byusing glucose oxidase method (GOD). Glucose was then converted into ethanol using Saccharomyces cerevisiae. Ethanol production was estimated by using spectrophotometer.The purposed project wasfocusing on bioethanol from the rice husk residues in which rice husk was pre-treated for the hydrolysis to produced maximum yield of sugar by selection of best pretreated method. And then sugars were converted into bioethanol by fermentation. Alkaline solutionswere remove lignin, while the acidic solutionswere remove hemicellulose. It was clearly showed in the graph.4.1 and 4.2 that best concentration of alkali 4% for the maximum yield of sugar was 50.4 mg/dl from the maximum 15g substrate of rice husk was used at 70˚C for 3hrs while in case of 4% acid treatment was also best for the maximum 42.6mg/dl sugar yield was obtainedat 70˚C for 3hrs.
According to statistical analysis and graphs 4.3 and 4.4 showed that maximum yieldi.e, 68.7 ml ethanolwas produced from the 15g rice husk when treated with 4%alkali,maximum temperature 35˚C with PH 6.0 and after 3days.While in case of 4%acid treatmentmaximum 63.7 ml ethanol was producedwhen maximum 15g rice husk was used at temperature 35˚C andPH with 6.0.after 3days.
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