The present study of 35 days duration was conducted at Poultry Research and Training
Centre (C-Block) Ravi Campus, University of Veterinary and Animal Sciences, Lahore to evaluate
the effect of mannan oligosaccharide supplementation on growth performance, carcass characteristics,
blood biochemistry and immune response in broilers. For this, a total of 360 commercial
broiler chickens were purchased from local hatchery and randomly divided into four treatment
groups having six replicated of 15 birds each, according to completely randomized design. Treatment
consisted four levels of MOS (0g/kg, 0.2g/kg, 0.4g/kg and 0.6g/kg). Weekly data were collected
regarding growth performance. At the end of experiment, three birds from each replicate
were randomly selected, after halal slaughtering carcass parameters were recorded. To find out the
effect of MOS on blood biochemistry and antibody titer against ND and IB, 5 ml blood sample
was harvested in marked test tubes from jugular vein of each bird at the time of slaughtering.
Serum samples were harvested by centrifugation (1500RPM) method and were stored at -20oC for
measuring blood glucose and cholesterol levels. Same serum samples were utilized to determine
the ND and IB antibodies titers. Collected data were analyzed through one-way ANOVA technique
using PROC GLM in SAS software. Supplementation of MOS level 0.6g/kg in the diet
of broilers resulted in improved growth performance, carcass characteristic, blood biochemical
profile and immune response in broilers. With respect to growth performance, significantly
improved body weight, weight gain and FCR were recorded in birds fed with 0.6g/kg
MOS. Regarding production performance indices, significantly better production efficiency
factor, point spread and performance index were recorded in birds having 0.6g/kg inclusion
of MOS in their diet. In the same way, 0.6 g/kg MOS significantly higher dressing, breast,
thigh yield, liver, heart, gizzard and intestinal weight percentage were recorded in birds
CHAPTER 6
SUMMARY
SUMMARY
36
having 0.6g/kg MOS in their diet. Regarding morphometric traits, significantly higher
keel length, shank length and intestinal length were recorded in birds supplemented 0.6g/kg
MOS in their diet. Significantly low glucose and cholesterol level were recorded in broilers
having 0.6g/kg MOS supplemented diet. Broilers fed MOS at 0.6/kg in their diet showed
better antibodies titers against ND and IB.