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Effect Of Age On Lipid Peroxidation Of Fresh And Frozen-Thawed Semen Of Nili-Ravi Buffalo Bulls

By: Sohail Ahmed (2014-VA-917) | Dr. Muhammad Irfan-ur-Rehman Khan.
Contributor(s): Dr. Sajid Iqbal | Dr. Muhammad Usman Mehmood | Dr. Muhammad Ijaz.
Material type: materialTypeLabelBookPublisher: 2016Description: 38p.Subject(s): TheriogenologyDDC classification: 2707-T Dissertation note: Buffalo spermatozoa are rich in polyunsaturated fatty acids and prone to lipid peroxidation. Malondialdehyde (MDA) is a byproduct of lipid peroxidation and causes irreversible damage to sperm structure and function. In buffalo, blood plasma MDA level increases with age. Therefore, we hypothesized that MDA level in buffalo bull semen will increase with age and will affect the semen quality. The objective of the study was to compare MDA level and quality of fresh and frozen-thawed semen in aged vs. young Nili-Ravi buffalo bulls. Single ejaculate was collected on weekly basis for four weeks from aged (13.6±1.0 years; n=3) and young (3.4±0.3 years; n=3) Nili-Ravi buffalo bulls. MDA level was estimated through thiobarbituric acid assay (TBA) in fresh and frozen-thawed semen. The quality of fresh and frozen-thawed semen was estimated through sperm motility, viability, DNA and acrosome integrity. MDA level (nmol/ml) did not differ (P>0.05) between aged vs. young bulls in fresh (2.3±0.2 vs. 2.9±0.7) and frozen-thawed (53.1±2.8 vs. 48.4±2.6) semen, respectively. In fresh semen, sperm motility and concentration did not differ (P>0.05) in aged vs. young bulls; however, the volume of fresh semen increased (P<0.05), while sperm viability and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed semen, sperm motility, viability, and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed vs. fresh semen, MDA level (nmol/ml) increased within young (48.4±2.6 vs. 2.3±0.2) and aged bulls (53.1±2.8 vs. 2.9±0.7), while motility and viability decreased (P<0.05) within the age groups. In conclusion, 1) lipid peroxidation (MDA) does not increase due to age in buffalo bull semen, and 2) freezing causes increase in lipid peroxidation irrespective of age and deteriorates semen quality of Nili-Ravi bulls.
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Buffalo spermatozoa are rich in polyunsaturated fatty acids and prone to lipid peroxidation. Malondialdehyde (MDA) is a byproduct of lipid peroxidation and causes irreversible damage to sperm structure and function. In buffalo, blood plasma MDA level increases with age. Therefore, we hypothesized that MDA level in buffalo bull semen will increase with age and will affect the semen quality. The objective of the study was to compare MDA level and quality of fresh and frozen-thawed semen in aged vs. young Nili-Ravi buffalo bulls. Single ejaculate was collected on weekly basis for four weeks from aged (13.6±1.0 years; n=3) and young (3.4±0.3 years; n=3) Nili-Ravi buffalo bulls. MDA level was estimated through thiobarbituric acid assay (TBA) in fresh and frozen-thawed semen. The quality of fresh and frozen-thawed semen was estimated through sperm motility, viability, DNA and acrosome integrity. MDA level (nmol/ml) did not differ (P>0.05) between aged vs. young bulls in fresh (2.3±0.2 vs. 2.9±0.7) and frozen-thawed (53.1±2.8 vs. 48.4±2.6) semen, respectively. In fresh semen, sperm motility and concentration did not differ (P>0.05) in aged vs. young bulls; however, the volume of fresh semen increased (P<0.05), while sperm viability and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed semen, sperm motility, viability, and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed vs. fresh semen, MDA level (nmol/ml) increased within young (48.4±2.6 vs. 2.3±0.2) and aged bulls (53.1±2.8 vs. 2.9±0.7), while motility and viability decreased (P<0.05) within the age groups. In conclusion, 1) lipid peroxidation (MDA) does not increase due to age in buffalo bull semen, and 2) freezing causes increase in lipid peroxidation irrespective of age and deteriorates semen quality of Nili-Ravi bulls.

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