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Molecularcharacterization And Antibiotic Resistance Pattern Of Pathogenic Eschericia Coli From Fancy Birds

By: Nageen sardar (2011-va-401) | Dr. Muhammad Asad Ali.
Contributor(s): Prof. Dr. Aftab Ahmed Anjum | Dr.WaseemShehzad.
Material type: materialTypeLabelBookPublisher: 2017Description: 65.Subject(s): microbiologyDDC classification: 2824-T Dissertation note: Colibacilosis is a disease which is really harmful as food borne illness and many population of earth has been suffering from it. If we do early diagnosis then it would be easy to identify the disease. E. coli is an opportunistic bacteria and it can easily cause disease in favorable conditions and may also act as zoonotic issue. So the persons having birds as pets in their houses should consider for the diagnosis. So, for this purpose the more accurate tests like polymerase chain reaction should be present. E. coli can cause serious risks for poultry industry and fancy birds. Colibacillosis is caused by Escherichia coli with serious threats related to food borne illness and most of world’s population is suffering from it. Fancy birds are kept in homes as pets, zoo and safari parks for entertainment of visitors. Fancy birds may be source of transmission of pathogenic E. coli to human beings. 150 cloacal samples were taken and the process of isolation, identification, antibiotic resistance pattern and molecular characterization was done for pathogenic E. coli. 150 samples from which 50 samples were from Pet Center University of Veterinary and Animal Sciences, Lahore, 50 samples were from Lahore Jalo Park and 50 samples were from Safari Park Lahore. Samples were shifted to enrichment media and then selective and differential media. After isolation microscopic characters was observed and biochemical identification was performed. Pathogenic isolates were collected and antibiotic resistance pattern was observed. After this DNA extraction was done, extracted DNA was observed on gel electrophoresis and then PCR was performed for toxigenic genes of E. coli.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2824-T (Browse shelf) Available 2824-T
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Colibacilosis is a disease which is really harmful as food borne illness and many population of earth has been suffering from it. If we do early diagnosis then it would be easy to identify the disease. E. coli is an opportunistic bacteria and it can easily cause disease in favorable conditions and may also act as zoonotic issue. So the persons having birds as pets in their houses should consider for the diagnosis. So, for this purpose the more accurate tests like polymerase chain reaction should be present. E. coli can cause serious risks for poultry industry and fancy birds.
Colibacillosis is caused by Escherichia coli with serious threats related to food borne illness and most of world’s population is suffering from it. Fancy birds are kept in homes as pets, zoo and safari parks for entertainment of visitors. Fancy birds may be source of transmission of pathogenic E. coli to human beings.
150 cloacal samples were taken and the process of isolation, identification, antibiotic resistance pattern and molecular characterization was done for pathogenic E. coli.
150 samples from which 50 samples were from Pet Center University of Veterinary and Animal Sciences, Lahore, 50 samples were from Lahore Jalo Park and 50 samples were from Safari Park Lahore. Samples were shifted to enrichment media and then selective and differential media. After isolation microscopic characters was observed and biochemical identification was performed.
Pathogenic isolates were collected and antibiotic resistance pattern was observed. After this DNA extraction was done, extracted DNA was observed on gel electrophoresis and then PCR was performed for toxigenic genes of E. coli.

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