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Effect Of Different Levels Of L-Valine On Production Performance, Serum Biochemistry And Illeal Digestibility Of Protein In Laying Hens (Lsl - Lite)

By: Usman Liaqat (2015-VA-1092) | Dr. Yasir Allah Ditta.
Contributor(s): Dr. Shafqat Nawaz Qaisrani | Dr. ShahzadAli.
Material type: materialTypeLabelBookPublisher: 2017Description: 59p.Subject(s): Animal NutritionDDC classification: 2857-T Dissertation note: Valine is a limiting branched amino acid in corn soya layer diet.It has both properties of glycogenic and ketogenic. It is also important in protein synthesis and precursor for many amino acids. A very little research has been conducted on levels of valine in layer strains globally, particularly research data is limited in Pakistan. So far, no trial has been conducted to find out the effect of different levels of valine for LSL lite in Pakistan. A total of 490 laying birds of LSL (LITE) strain of 33 weeks of age were selected and categorized into seven dietary treatments with different levels of Valine i.e. 0.70, 0.75, 0.78, 0.81, 0.84, 0.87 and 0.90 % of feed. All experimental diets were fed in mash form for 56 days of experimental period. Celite was added at rate of 2 % of feed for crude protein digestibility determination. At the end of experiment, two birds per replicate were randomly selected and slaughtered for the collection of blood and illeal digesta. Egg weight and egg production were calculated on daily basis. Three egg were selected from each replicate to measure egg quality parameters at end of each week. The egg quality parameters were calculated on weekly basis and body weight was measured at beginning, mid and end of the experimental period.At day 56, two birds from each replicate under different treatments were randomly selected and slaughtered to collect sample for ileal protein digestibility and blood for serum biochemistry. Ileal samples were stored at -20C. Serum samples were extracted and stored at -20C for measuring serum chemistry. Collected data were subjected to ONE WAY ANOVA under Complete Randomized Design. Duncan’s multiple range tests (DMR) were used to compare means. Statements of statistical significance are based on P < 0.05. Weekly collected data of egg quality parameters were analyzed by repeated measures SAS (version 9.1; SAS Inst. INc., Cary, NC). The current study shows significant influence of valine on production performance of laying hens. With increase in valine levels in the diet of laying hens, the egg production, FCR and feed intake significantly improved.Valine has significantly enhanced egg quality parameters i.e. egg weight, Haugh unit, egg yolk color, specific gravity, proportional yolk weight, proportional shell weight, eggyolk index and egg yolk to albumen ratio. Significant effects on total serum protein, serum albumin, immunity and ileal digestibility of proteins have been observedin laying hens. Following results shows that valine is limiting amino acid afterthreonine in diets devoid of animal protein in corn soya bean dietand must be given in diet for maximum production. Conclusion It can be concluded that valine is a limiting branched chain amino acid in corn soya layer diet. Supplementation of valine significantly increase egg production, improved FCR / dozen of laying hens. An increase in valine levels also significantly decreases the feed intake in laying hens which may be due to the antagonistic effects with BCCAA levels. Valine improved egg quality parameters (proportional yolk weight, proportional shell weight, egg weight, yolk color, specific gravity, yolk index and yolk to albumen ratio, Haugh unit) of laying hens. Influence of Valine has been observedon serum biochemistry (serum albumin, serum total protein and ND titer) of laying hens. Recommendations for the farmers Based on the current findings, 0.87 % valine is recommended level for maximum egg production at the age of 33-40 weeks of age with dig Val : dig Lys ratio of 1.04 : 1. Suggestions for the future researchers: 1. Same experiment can be done in broilers to assess growth performance, serum biochemistry and ileal digestibility of protein. 2. Future researchers can also apply this research in different layer strains.
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Valine is a limiting branched amino acid in corn soya layer diet.It has both properties of glycogenic and ketogenic. It is also important in protein synthesis and precursor for many amino acids. A very little research has been conducted on levels of valine in layer strains globally, particularly research data is limited in Pakistan. So far, no trial has been conducted to find out the effect of different levels of valine for LSL lite in Pakistan.
A total of 490 laying birds of LSL (LITE) strain of 33 weeks of age were selected and categorized into seven dietary treatments with different levels of Valine i.e. 0.70, 0.75, 0.78, 0.81, 0.84, 0.87 and 0.90 % of feed. All experimental diets were fed in mash form for 56 days of experimental period. Celite was added at rate of 2 % of feed for crude protein digestibility determination. At the end of experiment, two birds per replicate were randomly selected and slaughtered for the collection of blood and illeal digesta. Egg weight and egg production were calculated on daily basis. Three egg were selected from each replicate to measure egg quality parameters at end of each week. The egg quality parameters were calculated on weekly basis and body weight was measured at beginning, mid and end of the experimental period.At day 56, two birds from each replicate under different treatments were randomly selected and slaughtered to collect sample for ileal protein digestibility and blood for serum biochemistry. Ileal samples were
stored at -20C. Serum samples were extracted and stored at -20C for measuring serum chemistry. Collected data were subjected to ONE WAY ANOVA under Complete Randomized Design. Duncan’s multiple range tests (DMR) were used to compare means. Statements of statistical significance are based on P < 0.05. Weekly collected data of egg quality parameters were analyzed by repeated measures SAS (version 9.1; SAS Inst. INc., Cary, NC).

The current study shows significant influence of valine on production performance of laying hens. With increase in valine levels in the diet of laying hens, the egg production, FCR and feed intake significantly improved.Valine has significantly enhanced egg quality parameters i.e. egg weight, Haugh unit, egg yolk color, specific gravity, proportional yolk weight, proportional shell weight, eggyolk index and egg yolk to albumen ratio. Significant effects on total serum protein, serum albumin, immunity and ileal digestibility of proteins have been observedin laying hens. Following results shows that valine is limiting amino acid afterthreonine in diets devoid of animal protein in corn soya bean dietand must be given in diet for maximum production.
Conclusion
It can be concluded that valine is a limiting branched chain amino acid in corn soya layer diet. Supplementation of valine significantly increase egg production, improved FCR / dozen of laying hens. An increase in valine levels also significantly decreases the feed intake in laying hens which may be due to the antagonistic effects with BCCAA levels. Valine improved egg quality parameters (proportional yolk weight, proportional shell weight, egg weight, yolk color, specific gravity, yolk index and yolk to albumen ratio, Haugh unit) of laying hens. Influence of Valine has been observedon serum biochemistry (serum albumin, serum total protein and ND titer) of laying hens.
Recommendations for the farmers
Based on the current findings, 0.87 % valine is recommended level for maximum egg production at the age of 33-40 weeks of age with dig Val : dig Lys ratio of 1.04 : 1.



Suggestions for the future researchers:
1. Same experiment can be done in broilers to assess growth performance, serum biochemistry and ileal digestibility of protein.
2. Future researchers can also apply this research in different layer strains.

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