Production Of Polyhydroxybutyrate By Submerged Fermentation Using Agricultural By-Products
By: Zainab Bibi (2015-VA-802)
| Dr. Muhammad Tayyab.
Contributor(s): Dr. Shagufta Saeed | Prof. Dr. Aftab Ahmad Anjum.
Material type: 
BookPublisher: 2017Description: 49P.Subject(s): BiochemistryDDC classification: 2855-T Dissertation note: Increasing non-degradable waste on planet is the major environmental concern these days. Hence, there is an absolute need of “eco-friendly” plastics. Polyhydroxybutyrate (PHB) is the most popular biodegradable as well as eco-friendly polymer. However, high production cost of PHB is still a major problem in the commercialization of biodegradable plastics. Process economics revealed that the use of cheap and renewable carbon substrates such as agro-industrial wastes can account for 40-50% reduction in overall production cost.
In present study wheat bran, gram bran, rice bran, wheat straw and sesame oil cake were used to check PHB production using Azotobacter vinelandii NRRL-146641. For this purpose, 0.5ml inoculum was added in fermentation media and kept for incubation at 24-48 hrs. After incubation, both physical and chemical parameters such as (substrate water ratio, incubation time, inoculum volume, pH, agitation rate and nitrogen sources) were optimized. Optimized culture medium was centrifuged and obtained sediment was then used for analysis.
It was found that Azotobacter vinelandii in Rice bran contained medium gives maximum yield of PHB (248mg/100mL) at 8% substrate water ratio after 96 hours of incubation period (292mg/100mL), at 1.5 mL of volume of inoculum (304 mg/100mL), at pH 6.0 (316 mg/100mL), at 160 rpm agitation rate (416mg/100ml) at 0.3 % of yeast extract (446 mg/100mL) and 0.25% (436mg/100mL) of peptone. Obtained data was then analyzed by means of ONE-WAY ANOVA and through LSD test.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 2855-T (Browse shelf) | Available | 2855-T |
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Increasing non-degradable waste on planet is the major environmental concern these days. Hence, there is an absolute need of “eco-friendly” plastics. Polyhydroxybutyrate (PHB) is the most popular biodegradable as well as eco-friendly polymer. However, high production cost of PHB is still a major problem in the commercialization of biodegradable plastics. Process economics revealed that the use of cheap and renewable carbon substrates such as agro-industrial wastes can account for 40-50% reduction in overall production cost.
In present study wheat bran, gram bran, rice bran, wheat straw and sesame oil cake were used to check PHB production using Azotobacter vinelandii NRRL-146641. For this purpose, 0.5ml inoculum was added in fermentation media and kept for incubation at 24-48 hrs. After incubation, both physical and chemical parameters such as (substrate water ratio, incubation time, inoculum volume, pH, agitation rate and nitrogen sources) were optimized. Optimized culture medium was centrifuged and obtained sediment was then used for analysis.
It was found that Azotobacter vinelandii in Rice bran contained medium gives maximum yield of PHB (248mg/100mL) at 8% substrate water ratio after 96 hours of incubation period (292mg/100mL), at 1.5 mL of volume of inoculum (304 mg/100mL), at pH 6.0 (316 mg/100mL), at 160 rpm agitation rate (416mg/100ml) at 0.3 % of yeast extract (446 mg/100mL) and 0.25% (436mg/100mL) of peptone. Obtained data was then analyzed by means of ONE-WAY ANOVA and through LSD test.
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