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In Vitro Study Of The Effect Of Camphor On Electrophysiological Indices Of Isolated Omasum Epithelium In Calves

By: Uzma Yousaf (2015-VA-23) | Dr. Imtiaz Rabbani.
Contributor(s): Dr. Sajid Khan Tahir | Dr. Sana Ullah Iqbal.
Material type: materialTypeLabelBookPublisher: 2017Description: 44p.Subject(s): PhysiologyDDC classification: 2839-T Dissertation note: Agriculture is a backbone of Pakistan and livestock. Omasum is the third part in ruminant’s forestomach which absorbs the fluid.The endproduct of protein metabolism is ammonia and present NH4+ in ruminants and its reabsorption linked by non-selective cation channels.Addition of cation channel modulators such as camphor to diet can enhance absorption of nitrogen in the omasum. As it is suggest that ammonia transport is enhanced by essential oils. The purpose of this study is addition of camphor on isolated omasum epithelium of calves because electrophysiological changes primarily linked to the ammonia transport through activation of non-selective cation channels.Camphor may enhance short circuit currentand ammonia uptake inomasum epithelium of calves. Ten calves of uniform weight and age were slaughtered in a local slaughter house.Omasum will be removed from the abdominal cavity and cleaned in a standard transport buffer solution. Serosal layer of omasum were removed and mounted in the” ussing chamber”. Omasum epithelium after 20 minutes replaced with ammonia containing buffer solution and changes in bioelectric parameters were observed. Camphor in concentration of 1mM wasadded to mucosal side and electrophysiological parameters like Isc and Gt were observed. Moreover, pH of serosal side waschecked after camphor treatment at time intervals 20, 60 and 90 minutes respectively to assess ammonia transport. Camphor affected the electrophysiology of isolated omasum epithelium of calves by increasing short circuit current due to increase in ammonia concentrations in the omasum. Camphoraddition in 40mM NH4Cl containing solution significantly increased current (p < 0.05). ΔIsc was also significant between the groups containing 10 and 40mM NH4Cl containing solution. Change in potential difference was non significant after addition of camphor in both groups. Conductance increased significantly by increasing concentration of ammonia.Data were analyzed by using completely randomized ANOVA followed by post-hoc test used (p<0.05). Camphor treatment affects the electrophysiological indices of isolated omasal epithelium of calves and enhances ammonium transport across omasal epithelium increasing recycling of nitrogen. This may lead to efficient protein utilization by the animals.
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Veterinary Science 2839-T (Browse shelf) Available 2839-T
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Agriculture is a backbone of Pakistan and livestock. Omasum is the third part in ruminant’s forestomach which absorbs the fluid.The endproduct of protein metabolism is ammonia and present NH4+ in ruminants and its reabsorption linked by non-selective cation channels.Addition of cation channel modulators such as camphor to diet can enhance absorption of nitrogen in the omasum. As it is suggest that ammonia transport is enhanced by essential oils. The purpose of this study is addition of camphor on isolated omasum epithelium of calves because electrophysiological changes primarily linked to the ammonia transport through activation of non-selective cation channels.Camphor may enhance short circuit currentand ammonia uptake inomasum epithelium of calves.
Ten calves of uniform weight and age were slaughtered in a local slaughter house.Omasum will be removed from the abdominal cavity and cleaned in a standard transport buffer solution. Serosal layer of omasum were removed and mounted in the” ussing chamber”. Omasum epithelium after 20 minutes replaced with ammonia containing buffer solution and changes in bioelectric parameters were observed. Camphor in concentration of 1mM wasadded to mucosal side and electrophysiological parameters like Isc and Gt were observed. Moreover, pH of serosal side waschecked after camphor treatment at time intervals 20, 60 and 90 minutes respectively to assess ammonia transport.
Camphor affected the electrophysiology of isolated omasum epithelium of calves by increasing short circuit current due to increase in ammonia concentrations in the omasum.


Camphoraddition in 40mM NH4Cl containing solution significantly increased current (p < 0.05). ΔIsc was also significant between the groups containing 10 and 40mM NH4Cl containing solution. Change in potential difference was non significant after addition of camphor in both groups. Conductance increased significantly by increasing concentration of ammonia.Data were analyzed by using completely randomized ANOVA followed by post-hoc test used (p<0.05).
Camphor treatment affects the electrophysiological indices of isolated omasal epithelium of calves and enhances ammonium transport across omasal epithelium increasing recycling of nitrogen. This may lead to efficient protein utilization by the animals.

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