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Individual And Combined Effect Of Chicory Root Powder And Znso4 On Histology Of Intestine And Immune Organs In Broilers

By: Mamoona Din (2015-VA-1085) | Dr. Saima Masood.
Contributor(s): Dr. Hafsa Zaneb | Dr. Muhammad Shahbaz Yousaf.
Material type: materialTypeLabelBookPublisher: 2017Description: 75p.Subject(s): Anatomy and HistologyDDC classification: 2866-T Dissertation note: Phytogenic feed additives are plant derivatives which act as natural growth promoters and play crucial role in development of poultry health and production. Mineral supplementation also alters the immune status and gastrointestinal health and thus improves the performance in broilers. Thus intestinal and immune morphology is known to be modulated by nutritional interventions. Chicory is such a natural herb which acts as a prebiotic and zinc sulfate (ZnSO4) is a trace mineral which possesses antioxidant, immunomodulatory and antimicrobial properties Chicory root powder (CRP) and ZnSO4 have previously been used as growth promoters; however their combined effect on histology of broilers has not yet been evaluated. The present study was designed to report the individual and combined effect of CRP and ZnSO4 on histomorphometry of intestine, immune organs and some blood parameters in broilers. 150 one-day old broiler chicks were randomly divided into six groups: CONT, 2C, 4C, Zn60, 2C+Zn60, 4C+Zn60) with five replicates (n=5). Group-I was served as control and fed only basal diet (CONT), Group-II (BD + 2% CRP), Group-III (BD+ 4% CRP), Group IV (BD+ 60mg/kg feed ZnSO4), Group-V (BD+2% CRP+60mg/kg ZnSO4) and Group-VI (BD+4%CRP+60mg/kg ZnSO4). Birds were immunized against Newcastle disease, infectious bronchitis and infectious bursal disease. On day 35, two randomly selected birds from each replicate were slaughtered. Blood was collected in falcon tubes and centrifuged at 3000rpm for 10 minutes to obtain serum and triglycerides, cholesterol, HDL, LDL and VLDL were measured in serum. Three segments of small intestine (duodenum, jejunum, ileum) and caecum along with immune organs (cecal tonsils, thymus, spleen, bursa of fabricious) were excised and processed by paraffin embedding method and stained by H&E technique. Histological slides of intestine and immune organs were examined under light microscope (LABOMED® USA) for histomorphometric analysis. Data thus collected was analyzed by one-way ANOVA technique using SPSS. The means were compared using DMR test to measure significance at P<0.05. The results revealed that the level of triglycerides, LDL, total cholesterol and VLDL decreased significantly in individual 2% CRP and combination group of 2%CRP+ZnSO4 while cholesterol and HDL remained non-significant in all treatment groups as compared to control group. The length and weight of small intestine increased in significantly in 2% CRP and 2%CRP+ZnSO4 groups. The length of caecum increased in all treatment groups while weight of caecum was insignificant in all treatment groups as compared to control group. In duodenum, CD and MM decreased, VL/CD and ME increased significantly in 2% CRP and 2%CRP+ZnSO4 groups, while results of VL, VW, VSA, LP, EP were insignificant. In jejunum, VL, VL/CD, VSA and EP increased, CD decreased significantly in 2% CRP and 2%CRP+ZnSO4 groups. In ileum, VL/CD and EP increased significantly in 2% CRP and 2%CRP+ZnSO4 groups. The goblet cell count increased significantly in all intestinal segments in 2%CRP+ZnSO4 group. In caecal tonsils number, area and length of lymphatic nodules increased significantly in 2% CRP and 2%CRP+ZnSO4 groups. In thymus length of thymic lobules increased in 2% CRP and 4% CRP groups while width and area increased significantly in 4% CRP group. In spleen length and width of white pulp increased significantly in 2% CRP group. In bursa length, width and area of lymphatic nodules increased significantly in 2% CRP and 2%CRP+ZnSO4 groups, while thickness of mucosal folds was not affected significantly in all treatment groups. Dietary supplementation of chicory root powder and ZnSO4 may be useful in enhancing intestinal and immune organs microarchitecture and some blood parameters in broilers.
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Phytogenic feed additives are plant derivatives which act as natural growth promoters and play crucial role in development of poultry health and production. Mineral supplementation also alters the immune status and gastrointestinal health and thus improves the performance in broilers. Thus intestinal and immune morphology is known to be modulated by nutritional interventions. Chicory is such a natural herb which acts as a prebiotic and zinc sulfate (ZnSO4) is a trace mineral which possesses antioxidant, immunomodulatory and antimicrobial properties Chicory root powder (CRP) and ZnSO4 have previously been used as growth promoters; however their combined effect on histology of broilers has not yet been evaluated. The present study was designed to report the individual and combined effect of CRP and ZnSO4 on histomorphometry of intestine, immune organs and some blood parameters in broilers.
150 one-day old broiler chicks were randomly divided into six groups: CONT, 2C, 4C, Zn60, 2C+Zn60, 4C+Zn60) with five replicates (n=5). Group-I was served as control and fed only basal diet (CONT), Group-II (BD + 2% CRP), Group-III (BD+ 4% CRP), Group IV (BD+ 60mg/kg feed ZnSO4), Group-V (BD+2% CRP+60mg/kg ZnSO4) and Group-VI (BD+4%CRP+60mg/kg ZnSO4). Birds were immunized against Newcastle disease, infectious bronchitis and infectious bursal disease.
On day 35, two randomly selected birds from each replicate were slaughtered. Blood was collected in falcon tubes and centrifuged at 3000rpm for 10 minutes to obtain serum and triglycerides, cholesterol, HDL, LDL and VLDL were measured in serum. Three segments of small intestine (duodenum, jejunum, ileum) and caecum along with immune organs (cecal tonsils, thymus, spleen, bursa of fabricious) were excised and processed by paraffin embedding method and stained by H&E technique. Histological slides of intestine and immune organs were examined under light microscope (LABOMED® USA) for histomorphometric analysis. Data thus collected was analyzed by one-way ANOVA technique using SPSS. The means were compared using DMR test to measure significance at P<0.05.
The results revealed that the level of triglycerides, LDL, total cholesterol and VLDL decreased significantly in individual 2% CRP and combination group of 2%CRP+ZnSO4 while cholesterol and HDL remained non-significant in all treatment groups as compared to control group. The length and weight of small intestine increased in significantly in 2% CRP and 2%CRP+ZnSO4 groups. The length of caecum increased in all treatment groups while weight of caecum was insignificant in all treatment groups as compared to control group. In duodenum, CD and MM decreased, VL/CD and ME increased significantly in 2% CRP and 2%CRP+ZnSO4 groups, while results of VL, VW, VSA, LP, EP were insignificant. In jejunum, VL, VL/CD, VSA and EP increased, CD decreased significantly in 2% CRP and 2%CRP+ZnSO4 groups. In ileum, VL/CD and EP increased significantly in 2% CRP and 2%CRP+ZnSO4 groups. The goblet cell count increased significantly in all intestinal segments in 2%CRP+ZnSO4 group.
In caecal tonsils number, area and length of lymphatic nodules increased significantly in 2% CRP and 2%CRP+ZnSO4 groups. In thymus length of thymic lobules increased in 2% CRP and 4% CRP groups while width and area increased significantly in 4% CRP group. In spleen length and width of white pulp increased significantly in 2% CRP group. In bursa length, width and area of lymphatic nodules increased significantly in 2% CRP and 2%CRP+ZnSO4 groups, while thickness of mucosal folds was not affected significantly in all treatment groups.
Dietary supplementation of chicory root powder and ZnSO4 may be useful in enhancing intestinal and immune organs microarchitecture and some blood parameters in broilers.

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