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Basic Cell Culture Protocols

By: Helgason, Cheryl D.
Contributor(s): Helgason, Cheryl D | Miller, Cindy L.
Material type: materialTypeLabelBookSeries: Publisher: New Jersey: Humana Press, 2005Edition: 3rd ed. 2005.Description: 384 p.ISBN: 1588295451 (paperback); 9781588295453 (paperback).Subject(s): Cell culture | Tissue culture | Cytology | Life sciencesDDC classification: 571.638 Helgason 20026 V.290,3rd 2005 Genetics Summary: In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).
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Item type Current location Collection Call number Status Date due Barcode Item holds
Books Books UVAS Library
Genetics
Veterinary Science 571.638 Helgason 20026 3rd.Vol.290 2005 Genetics (Browse shelf) Available 20026
Total holds: 0
Browsing UVAS Library Shelves , Shelving location: Genetics , Collection code: Veterinary Science Close shelf browser
571.629 Cossart 19997 2nd 2005 Genetics Cellular Microbiology 571.638 Bonifacino 17216 1st 2004 Genetics Short Protocols in Cell Biology 571.638 Bray 27500 1st 2013 Genetics Stem Cell Culture 571.638 Helgason 20026 3rd.Vol.290 2005 Genetics Basic Cell Culture Protocols 571.6381 Barry 27497 1st 2013 Genetics Recent Advances in Animal Cell and Tissue Culture 571.6381 Davis 27425 2nd 2011 Genetics Animal Cell Culture : Essential Methods 571.6381 Freshney 17743 5th 2005 Genetics Culture of Animal Cells / 5th ed

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

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