Bioconversion Of Agriculture Waste To Polyhydroxybutyrate Through Solid State Fermentation With Bacillus Thuringiensis
By: Hafsa Yasmin(2015-VA-1065)
| Dr.Abu Saeed Hashmi.
Contributor(s): Dr.Shagufta Saeed | Dr.Shahbaz yousaf.
Material type: 
BookPublisher: 2017Description: 60p.Subject(s): BiochemistryDDC classification: 2964-T Dissertation note: Polyhydroxybutyrate (PHB) is a biopolymer,it can be used as a biodegradable thermoplastic material for waste management strategies.it can be produced by various microorganism bacterium, Bacillus thuringiensis accumulates PHB as intracellular granules Inside their cells in response to physiological stress such as excess of carbon sources and limitation of nutrients e.g. nitrogen and phosphorus etc. This research work PHB productionwas attempted from agriculture wastes like wheat bran, rice polishing and corn stover through solid state fermentation through optimization of different parameters like substrate water ratio, incubation period, volume of inoculum, inorganic salts concentrations, addition of molasses and corn steep liquor. A parent strain of Bacillus thuringiensis was maintained on nutrient agar plate. Fermentation media containing wheat bran, rice polishing and corn stover as substrates were used to check the production of PHB for the selected bacteria. Inoculum 0.5 mL was added into sterilized fermentation media and incubated for 0-96 hours. Thereafter growth media were harvested, culture was centrifuged. The extraction, determination and identification of PHB were carried out from the pellet.
It was found that Bacillus thuringiensisgave the maximum PHB yield (270mg/100g) with 10 mL of basal medium containing rice polishing, while the wheat bran produced (210mg/100g) at 72 hours and corn stover produced yield (130mg/100g) at 48 hours.Rice polishing gave the maximum PHB production yield (2000mg/100g) at 1mL of inoculum in the presence of optimized condition of 0.175% of KH2PO4.2H2O, 0.125% MgSO4.7H20, 0.75% urea, 1% corn steep liquor respectively.
In this study Bacillus thuringiensis produced higher yield of PHB using rice polishing as compared to wheat bran and corn stover.
So, it is concluded that PHB produced in this work can be used in various industries like pharmaceutics, food industry and also in medical field, it will also be helpful to reduce the pollution caused by other synthetic plastics.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 2964-T (Browse shelf) | Available | 2964-T |
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Polyhydroxybutyrate (PHB) is a biopolymer,it can be used as a biodegradable thermoplastic material for waste management strategies.it can be produced by various microorganism bacterium, Bacillus thuringiensis accumulates PHB as intracellular granules Inside their cells in response to physiological stress such as excess of carbon sources and limitation of nutrients e.g. nitrogen and phosphorus etc. This research work PHB productionwas attempted from agriculture wastes like wheat bran, rice polishing and corn stover through solid state fermentation through optimization of different parameters like substrate water ratio, incubation period, volume of inoculum, inorganic salts concentrations, addition of molasses and corn steep liquor. A parent strain of Bacillus thuringiensis was maintained on nutrient agar plate. Fermentation media containing wheat bran, rice polishing and corn stover as substrates were used to check the production of PHB for the selected bacteria. Inoculum 0.5 mL was added into sterilized fermentation media and incubated for 0-96 hours. Thereafter growth media were harvested, culture was centrifuged. The extraction, determination and identification of PHB were carried out from the pellet.
It was found that Bacillus thuringiensisgave the maximum PHB yield (270mg/100g) with 10 mL of basal medium containing rice polishing, while the wheat bran produced (210mg/100g) at 72 hours and corn stover produced yield (130mg/100g) at 48 hours.Rice polishing gave the maximum PHB production yield (2000mg/100g) at 1mL of inoculum in the presence of optimized condition of 0.175% of KH2PO4.2H2O, 0.125% MgSO4.7H20, 0.75% urea, 1% corn steep liquor respectively.
In this study Bacillus thuringiensis produced higher yield of PHB using rice polishing as compared to wheat bran and corn stover.
So, it is concluded that PHB produced in this work can be used in various industries like pharmaceutics, food industry and also in medical field, it will also be helpful to reduce the pollution caused by other synthetic plastics.
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