Gastrointestinal Nematodes (GINs) infection is one of the main components to face against well-organized feed conversion. These infections not only cause production losses as well as increase the management and treatment cost of animals in Pakistan. They are extensive in prevalence and are found in tropical and subtropical areas of the world and have anxious the production probable by causing numberless deaths and indirect reasonable fatalities. (Terefe et al. 2012). Helminthes, seriously condensed the small ruminant production system and are responsible for illness in both warmer and colder parts of the world (Kumsa and Abebe 2009). Gastro-intestinal nematodes (GINs) highly affect the production of sheep owed to decrease in desire for food, bad body condition and score (BCS), anemia, hypo-proteinaemia, impaired digestive absorptive efficiency, many other pathogenic impediments and even result in mortalities (FAO, 1974; Barger, 1982; Steel and Symons, 1982).
Both ram and ewe of different age and breed fulfilling our criteria were selected for the designed research trial. All included, sheep were either directly from rectum or fresh passed feces were sampled at random. Each fecal sample was examined microscopically in order to estimate worm burden by modified McMaster technique (Coles, 1992). Sheep found positive for gastro-intestinal parasites were selected for the evaluation of anthelmintics against Oxfendazole, Levamisole and Wormafax (Herbel product). Furthermore, 2nd and 3rd samples were colledted at the end of second and third week of drenching, respectively. The dosages of anthelmintic administered are described in table 3.1.
After collection, screening of all samples was done through microscope and floatation technique followed by quantitative examination through McMaster techniques (Soulsby, 1982; Zajac and Conboy, 2006) and EPG was recorded. Before and after treatment EPG of each group was then compared, % efficacy of administered drugs were measured and significance of pretreatment and post treatment EPG within group were analyzed through one way ANOVA followed by Tukey post hoc test (P<0.05). Mean efficacy of Oxfendazole, levamisole and wormafax was found to be 87.43%, 98.2%, 99.6% respectively.
Result of our designed project show that resistance have been developed against oxfendazole and levamisole in Haemonchuscontortus as per the guidelines given by WAAVP which is 95% efficacy herbal product wormafax is still effective.