Sero-Epidemiological Survey Of Bovine Viral Diarrhea In Lahore Using Agar Gel Diffusion Technique, With An Attempt On Isolation of Virus
By: Fahim Ahmad, Bhatti
| Ata Ur -Rehman Rizvi.
Contributor(s): Muhammed | Muhammed Amin Sheikh | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1989Subject(s): Department of MicrobiologyDDC classification: 0167,T Dissertation note: The seroloical study was carried out on one thousand serum samples collected randomly from abbatoirs at Lahore city. Of these serum samples 645 were from buffaloes while 55 were of cattle origin. This study was undertaken to study- the seroepidemiology off bovine viral diarrhea in and around Lahore city. Agar gel precipitation test was used for detecting antibodies in the Sera of animals. The primary bovine kidney cell cultures were used for culturing the bovine viral diarrhea virus and the antigen for immuno-diffusion tests was prepared from the infected cells.
An overall incidence of 8.6% was revealed by the agar-gel immuno-diffusion tests. The tests showed 914% buffaloes as having antibodies against bovine viral diarrhea, while '76% of the cattle showed positive results.
The influence of various epidemiologi- cal factors was proven. Higher prevalence rate was observed in the buffaloes as compared to the cattle. The age group of the animals was also found to be one of the factors affecting the distribution of bovine viral diarrhea antibodies, as maximum number of reactors were detected in the 10 years and above age group through agar-gel precipitation tests. Attempts were made on the isolation of virus on primary bovine kidney cell cultures, but were not successful.
The results of the study revealed few animals having precipitating antibodies in their sera. It shows that the disease is not widespread amongst the animals in our area; bit 8till calls for an emergent response from
experts and relevant agencies for mass scale surveys to get a better understanding of the disease and its epidemiology.
Bovine viral diarrhea - Mucosal disease complex has a great economic impact on the livestock industry in general and on the dairy and breeding programmes in particular. As Pakistan is a developing country and the economy relies heavily on the agriculture and livestock resources, therefore, control measures should be adopted without delay to have a proper check on this malady before it spreads to an un-controlable extent.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0167,T (Browse shelf) | Available | 0167,T |
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The seroloical study was carried out on one thousand serum samples collected randomly from abbatoirs at Lahore city. Of these serum samples 645 were from buffaloes while 55 were of cattle origin. This study was undertaken to study- the seroepidemiology off bovine viral diarrhea in and around Lahore city. Agar gel precipitation test was used for detecting antibodies in the Sera of animals. The primary bovine kidney cell cultures were used for culturing the bovine viral diarrhea virus and the antigen for immuno-diffusion tests was prepared from the infected cells.
An overall incidence of 8.6% was revealed by the agar-gel immuno-diffusion tests. The tests showed 914% buffaloes as having antibodies against bovine viral diarrhea, while '76% of the cattle showed positive results.
The influence of various epidemiologi- cal factors was proven. Higher prevalence rate was observed in the buffaloes as compared to the cattle. The age group of the animals was also found to be one of the factors affecting the distribution of bovine viral diarrhea antibodies, as maximum number of reactors were detected in the 10 years and above age group through agar-gel precipitation tests. Attempts were made on the isolation of virus on primary bovine kidney cell cultures, but were not successful.
The results of the study revealed few animals having precipitating antibodies in their sera. It shows that the disease is not widespread amongst the animals in our area; bit 8till calls for an emergent response from
experts and relevant agencies for mass scale surveys to get a better understanding of the disease and its epidemiology.
Bovine viral diarrhea - Mucosal disease complex has a great economic impact on the livestock industry in general and on the dairy and breeding programmes in particular. As Pakistan is a developing country and the economy relies heavily on the agriculture and livestock resources, therefore, control measures should be adopted without delay to have a proper check on this malady before it spreads to an un-controlable extent.
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