Isolation Of Infectious Bursal Disease (Gumboro Disease) Virus From Infected Birds Based Upon Lesions In Embryonated Eggs
By: Khalid Iqbal
| Muhammed Naeem.
Contributor(s): Sheikh Muhammed Amin | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1988Subject(s): Department of MicrobiologyDDC classification: 0183,T Dissertation note: A number of 30 bursae of fabricius were collected from the birds suspected for Infectious Bursal Disease, brought to the Disease Diagnostic Section, Poultry Development Centre (P.R.I.), Rawalpindi and Animal Sciences Institute, National Agriculture Research Council, Islamabad. Each sample was processed and inoculated through allantoic sac route in five embryonated eggs of 9 to 11 days. Eggs showing embryo deaths between 48 hours post inoculatiom were discarded as it was non specific embryo mortality. Five embryonated eggs were kept as control. Five serial passages were done to check the embryo mortality pattern.
Out of 30 samples, nine samples showed a typical Infectious Bursal Disease embryo mortality pattern i.e. 100% mortality at first passage, 30 to 33% mortality at second passage and 0% in subsequent passages. Seven samples showed a positive reaction in Agar Gel Precipitation Test out of nine samples. The incidence of Infectious Bursal Disease was 3 .3% in and around Rawalpindi.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0183,T (Browse shelf) | Available | 0183,T |
A number of 30 bursae of fabricius were collected from the birds suspected for Infectious Bursal Disease, brought to the Disease Diagnostic Section, Poultry Development Centre (P.R.I.), Rawalpindi and Animal Sciences Institute, National Agriculture Research Council, Islamabad. Each sample was processed and inoculated through allantoic sac route in five embryonated eggs of 9 to 11 days. Eggs showing embryo deaths between 48 hours post inoculatiom were discarded as it was non specific embryo mortality. Five embryonated eggs were kept as control. Five serial passages were done to check the embryo mortality pattern.
Out of 30 samples, nine samples showed a typical Infectious Bursal Disease embryo mortality pattern i.e. 100% mortality at first passage, 30 to 33% mortality at second passage and 0% in subsequent passages. Seven samples showed a positive reaction in Agar Gel Precipitation Test out of nine samples. The incidence of Infectious Bursal Disease was 3 .3% in and around Rawalpindi.
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