Studies On The Isolation And Characterization Of Staphylococus Aureus From Milk With Special Reference
By: Samad Khan, A
| M.Naeem.
Contributor(s): M.Saeed | S.A.Basra | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1984Subject(s): Department of MicrobiologyDDC classification: 0188,T Dissertation note: Staphylococcus aureus being an umbiquitous potential pathogen of man and animal, can contaminate the milk by either of the two sources. The present study was designed to find the source of origin of the isolated strains on the basis of their haemolytic behaviour. Thus, from 200 raw market milk samples Staphylococcus aureus was isolated from 179 samples. Out of these 179 strains 100 were randomly selected for the study of their haemolytic pattern on sheep bloc4and rabbit blood agar plates incubated aerobically.
It was found that 27 strains were Alpha-haemolytic, 46 were beta-haemolytic whereas 27 strains showed a mixed behaviour of alpha-beta type. It was concluded that both man and animal were the source of contamination of milk, the percentage of animal strains being higher than the human.
The Standard Plate Count showed that out of 200 samples 90 (45%) had a count of grade B raw milk, while 110 samples (55%) had a bacterial count of grade C raw milk. 126 (63%) samples out of 200 had a Coliform count of more than 10, while 74 samples (37%) had a count of less than 10 coliforms per ml of raw market milk tested. There was a positive correlation between high bacterial count and the presence of Staphylococcus aureus in milk.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
|
UVAS Library Thesis Section | Veterinary Science | 0188,T (Browse shelf) | Available | 0188,T |
Staphylococcus aureus being an umbiquitous potential pathogen of man and animal, can contaminate the milk by either of the two sources. The present study was designed to find the source of origin of the isolated strains on the basis of their haemolytic behaviour. Thus, from 200 raw market milk samples Staphylococcus aureus was isolated from 179 samples. Out of these 179 strains 100 were randomly selected for the study of their haemolytic pattern on sheep bloc4and rabbit blood agar plates incubated aerobically.
It was found that 27 strains were Alpha-haemolytic, 46 were beta-haemolytic whereas 27 strains showed a mixed behaviour of alpha-beta type. It was concluded that both man and animal were the source of contamination of milk, the percentage of animal strains being higher than the human.
The Standard Plate Count showed that out of 200 samples 90 (45%) had a count of grade B raw milk, while 110 samples (55%) had a bacterial count of grade C raw milk. 126 (63%) samples out of 200 had a Coliform count of more than 10, while 74 samples (37%) had a count of less than 10 coliforms per ml of raw market milk tested. There was a positive correlation between high bacterial count and the presence of Staphylococcus aureus in milk.
There are no comments for this item.