Superovulation In Jersey Cows Under Local Environment
By: Qaiyum Naveed
| Tayyab Raheel.
Contributor(s): Muhammid | Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1991Subject(s): Department of TheriogenologyDDC classification: 0234,T Dissertation note: Reproductive capacity of a valuable animal can be increased through embryo transfer technology. Superovulation is a pre-requisite for a successful embryo transfer. In the present project an attempt has been made to study the superovulatory response of purebred Jersey cows under the climatic conditions of Pakistan.
Twenty cyclic Jersey cows were divided into two equal groups; A and B, each comprising 10 animals. Each cow in Group A was injected 2500 IU PMSG on day 9 post - estrus (estrus = day 0) and was followed 48 hours later by 15 mg of PGF2 alpha. Group B served as control and cows in this group were injected with normal saline only on day 9 post estrus and 48 hours later PGF2 alpha was injected. Cows were inseminated with frozen bull semen 12 hours after the start of estrus Insemination was repeated once again at an interval of 12 hours. Embryos were recovered nonsurgically on day 7 post - insemination
The mean interval from PGF2 alpha administration till the time of heat expression did not differ between the two groups and was 37.34 ± 0.41 and 37.73 ± 0.15 hours in Groups A and B, respectively. In Group A mean number of corpora lutea (5.3 ± 1.55) and palpable follicles (5.4 ± 0.93) was significantly (P <0.05) greater than those in Group B (CL 1.00 ± 0.00 and follicle 0.5 ± 0.16). No statistical difference was noted in the percentage recovery of flushing media between Groups A (76.7 ± 2.0) and B (81.0 ± 1.3). A total of 29 embryos were recovered in Group A out of 10 flushings. Most of the embryos recovered were either compact morulae or expanded blastocysts and some were degenerated. From Group 13 no embryo/ova could be recovered. The superovulatory response of the Jersey cows maintained under local climatic conditions seems to be a little less when compared with that of the cows which are maintained under exotic (temperate) environment.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0234,T (Browse shelf) | Available | 0234,T |
Reproductive capacity of a valuable animal can be increased through embryo transfer technology. Superovulation is a pre-requisite for a successful embryo transfer. In the present project an attempt has been made to study the superovulatory response of purebred Jersey cows under the climatic conditions of Pakistan.
Twenty cyclic Jersey cows were divided into two equal groups; A and B, each comprising 10 animals. Each cow in Group A was injected 2500 IU PMSG on day 9 post - estrus (estrus = day 0) and was followed 48 hours later by 15 mg of PGF2 alpha. Group B served as control and cows in this group were injected with normal saline only on day 9 post estrus and 48 hours later PGF2 alpha was injected. Cows were inseminated with frozen bull semen 12 hours after the start of estrus Insemination was repeated once again at an interval of 12 hours. Embryos were recovered nonsurgically on day 7 post - insemination
The mean interval from PGF2 alpha administration till the time of heat expression did not differ between the two groups and was 37.34 ± 0.41 and 37.73 ± 0.15 hours in Groups A and B, respectively. In Group A mean number of corpora lutea (5.3 ± 1.55) and palpable follicles (5.4 ± 0.93) was significantly (P <0.05) greater than those in Group B (CL 1.00 ± 0.00 and follicle 0.5 ± 0.16). No statistical difference was noted in the percentage recovery of flushing media between Groups A (76.7 ± 2.0) and B (81.0 ± 1.3). A total of 29 embryos were recovered in Group A out of 10 flushings. Most of the embryos recovered were either compact morulae or expanded blastocysts and some were degenerated. From Group 13 no embryo/ova could be recovered. The superovulatory response of the Jersey cows maintained under local climatic conditions seems to be a little less when compared with that of the cows which are maintained under exotic (temperate) environment.
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