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Isolation & Characterization Of Newcastle Disease Virus Strains In Poultry

By: Shazia Afzal | Sheikh Muhammed Amin.
Contributor(s): Asif Rabbani | Muhammed Ajmal | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 1992Subject(s): Department of MicrobiologyDDC classification: 0242,T Dissertation note: A study was undertaken to isolate and characterize Newcastle Disease Virus Strains involved in field outbreaks. A total of 1000 diseased/dead birds ( broilers, layers arid non-discript birds ) were examined for this purpose. The isolation of the virus was attempted from composite samples comprising lungs, liver, spleen, brain and proventriculus of the suspected birds, which was inoculated in embryonated eggs through allontoic cavity route. Out of the total birds examined only 6(0.06%) yielded ND Virus. All the strains present in allontoic fluid gave a positive reaction to spot haemagglutination test, on fowl RBCs. The isolates were confirmed as Newcastle disease pathogen through a positive reaction to haemagglutination inhibition test conducted with known positive sera against Newcastle disease. Various value values obtained for mean death time (MDT) were 48, 60, 70, 72, 48 and 62 hours for isolates. No. 1, 2, 3, 4, 5, and £ respectively.. Values of intracerebral pathogencity index far isolated No. 1, 2, 3, 4, 5, and 6 were 1.71, 1.68, 1.58, 1.46, 1.7 and 1.65 respectively. The intravenous pathogenecity index varies recorded were 230, 200, 1.91, 1.88, 2.08 and 1.93 for isolate No. 1, 2, 3, 4, 5, and 6 respectively.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
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Veterinary Science 0242,T (Browse shelf) Available 0242,T
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A study was undertaken to isolate and characterize Newcastle Disease Virus Strains involved in field outbreaks.

A total of 1000 diseased/dead birds ( broilers, layers arid non-discript birds ) were examined for this purpose. The isolation of the virus was attempted from composite samples comprising lungs, liver, spleen, brain and proventriculus of the suspected birds, which was inoculated in embryonated eggs through allontoic cavity route. Out of the total birds examined only 6(0.06%) yielded ND Virus. All the strains present in allontoic fluid gave a positive reaction to spot haemagglutination test, on fowl RBCs. The isolates were confirmed as Newcastle disease pathogen through a positive reaction to haemagglutination inhibition test conducted with known positive sera against Newcastle disease.

Various value values obtained for mean death time (MDT) were 48, 60, 70, 72, 48 and 62 hours for isolates.

No. 1, 2, 3, 4, 5, and £ respectively.. Values of intracerebral pathogencity index far isolated No. 1, 2, 3, 4, 5, and 6 were 1.71, 1.68, 1.58, 1.46, 1.7 and 1.65 respectively. The intravenous pathogenecity index varies recorded were 230, 200, 1.91, 1.88, 2.08 and 1.93 for isolate No. 1, 2, 3, 4, 5, and 6 respectively.

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