Studies On Isolation, Identification And Pathogenicity Of Bacteria Isolated From The Respiratory Tract Of Cattle And Buffaloes At Lahore Abattoir
By: Yousaf, M | Muhammed akram Muneer.
Contributor(s): Ata-Ur- Rizvi | Imtiaz Hussain Khan | Faculty of Veterinary Sciences.
Material type: BookPublisher: 1992Subject(s): Department of MicrobiologyDDC classification: 0253,T Dissertation note: total of 1200 samples consisting of 300 nasal, 300 tracheal, 300 bronchial swabs and 300 pieces of lung tissue from 150 randomly selected cows and buffaloes (75 from each species) were collected from abattoir of Lahore Corporation. Of the total samples 702 isolates were isolated and identified on the basis of their morphological, cultural, and biochemical characteristics. The isolates included Pdsteurellp multocida 89 strains, (45 strains from buffaloes and 44 strains from cows), Pasteurella haemolvtica 98 strains (59 from buffaloes and 39 strains from cows), Corynebacterium ovocenes 76 strains (54 strains from buffaloes and 22 strains from cows), Escherichia coli 146 strains (80 strains from buffaloes and 66 strains from cow), Staphylococcus epidermidis 152 strains (75 strains from buffaloes and 77 strains from cows), Bacillus subtilit 135 strains (67 strains from buffaloes and 68 strains from cows), and Mycobacterium bovis 6 strains (2 strains from buffaloes and j strains from cattle). The isolation percentage of Pasteurella. multocida was 19.66% in buff aloes and 13% in cattle, 15% Pasteurella haemolytica was present in buffaloes and 14.66% Pasteurella haemolvtica in cows. 18% and 7.33% Corvnebacterium pyogenes could be isolated from buffaloes arid cattle respectively. 26.66% and 22% Escherictiia coli was isolated from buffaloes and cattle respectively. From buffaloes and cows 25 and 25.66% of Staohvlococcus epidermidis was isolated respectively. Bacillus subtilis was 22.33% and 22.66% in buffaloes and cows respectively 2.66% and 5.33% Mvcobacterium bovis was isolated from buffaloes and cattle respectively. There was small difference in the number of isolates from buffaloes and cattle. Pasteurella haemolytica, Staphyloicoccus epidermidis and Bacillus subtilis proved to be nonpathogenic while Pagsteurella multocida, Corynebacterium pyogenes ai.d Escherichia coli caused l00, 50 and 59 percent mortality on experimental inoculation. Mycobacterum bovis was non pathogenic for mice. However upon intraperitoneal inoculation in rabbit it caused 57% mortality.Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis | UVAS Library Thesis Section | Veterinary Science | 0253,T (Browse shelf) | Available | 0253,T |
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total of 1200 samples consisting of 300 nasal, 300 tracheal, 300 bronchial swabs and 300 pieces of lung tissue from 150 randomly selected cows and buffaloes (75 from each species) were collected from abattoir of Lahore Corporation. Of the total samples 702 isolates were isolated and identified on the basis of their morphological, cultural, and biochemical characteristics. The isolates included Pdsteurellp multocida 89 strains, (45 strains from buffaloes and 44 strains from cows), Pasteurella haemolvtica 98 strains (59 from buffaloes and 39 strains from cows), Corynebacterium ovocenes 76 strains (54 strains from buffaloes and 22 strains from cows), Escherichia coli 146 strains (80 strains from buffaloes and 66 strains from cow), Staphylococcus epidermidis 152 strains (75 strains from buffaloes and 77 strains from cows), Bacillus subtilit 135 strains (67 strains from buffaloes and 68 strains from cows), and Mycobacterium bovis 6 strains (2 strains from buffaloes and j strains from cattle).
The isolation percentage of Pasteurella. multocida was 19.66% in buff aloes and 13% in cattle, 15% Pasteurella haemolytica was present in buffaloes and 14.66% Pasteurella haemolvtica in cows. 18% and 7.33% Corvnebacterium pyogenes could be isolated from buffaloes arid cattle respectively. 26.66% and 22% Escherictiia coli was isolated from buffaloes and cattle respectively. From buffaloes and cows 25 and 25.66% of Staohvlococcus epidermidis was isolated respectively. Bacillus subtilis was 22.33% and 22.66% in buffaloes and cows respectively 2.66% and 5.33% Mvcobacterium bovis was isolated from buffaloes and cattle respectively.
There was small difference in the number of isolates from buffaloes and cattle. Pasteurella haemolytica, Staphyloicoccus epidermidis and Bacillus subtilis proved to be nonpathogenic while Pagsteurella multocida, Corynebacterium pyogenes ai.d Escherichia coli caused l00, 50 and 59 percent mortality on experimental inoculation. Mycobacterum bovis was non pathogenic for mice. However upon intraperitoneal inoculation in rabbit it caused 57% mortality.
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