Toxigenic Properties Of Staphylococcus Aureus From Human And Animal Origin
By: Tayyaba Rashid
| Ata-Ur-Rehman Rizvi.
Contributor(s): Manzoor | Muhammed Ajmal | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1984Subject(s): Department of MicrobiologyDDC classification: 0288,T Dissertation note: A total number of hundred strains of Staphylococcus aureus including 50 human and 50 horse wounds strains, were isolated on Staphylococcus medium No.110. The isolated strains were maintained on nutrient agar slants. The strains of Staphylococcus aureus were examined for the production of coagulase, fibrinolysin, alpha haemolysin,beta haemolysin and delta toxin. The isolated strains were coagulase positive but a variable coagulase activity against the plasma of human, sheep and rabbit. Human plasma gave better results than rabbit and sheep plasma. brinolytic activity of Stapylococcus aureus was tested in oxalated plasma of human, sheep and rabbit. Strains isolated from human surgical wounds were found to be more fibrinolytic as compared to horse isolates. Human and rabbit plasma were a better indicators for checking fibrinolytic activity. None of the isolated strains showed fibrinolytic activity against sheep plasma.
The isolates of Stphlococcus aureus were cultured for alpha haernolysin production in nutrient broth culture. Alpha toxin was purified and its haemolysin activity was determined qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Maximum haemolytic activity was observed against rabbit erythrocytes. Out of hundred strains, sixty-eight produced alpha haemolysin including 45 from human and 23 from horse wounds.
The isolates of Staphylococcus aureus were cultured in semi-solid Heart infusion agar for the production of eta haemolysin. This toxin was purified and its haemolytic activity was determined qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Naximum haemolytic activity was observed against sheep erythrocytes. Out of the hundred strains, beta haemolysin was produced by sixty-two strains including 15 from human and 47 from horses.
The isolates of Staphylococcus aureus were cultured in Brain Heart infusion agar for .the production of delta toxin. This toxin was purified and its haernolytic activity was observed qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Maximum haemolytic activity of dlta toxin was observed against human erythrocytes. Out of the hundred strains, seventy-one produced delta toxin including 43 from human and 28 from horses.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0288,T (Browse shelf) | Available | 0288,T |
A total number of hundred strains of Staphylococcus aureus including 50 human and 50 horse wounds strains, were isolated on Staphylococcus medium No.110. The isolated strains were maintained on nutrient agar slants. The strains of Staphylococcus aureus were examined for the production of coagulase, fibrinolysin, alpha haemolysin,beta haemolysin and delta toxin. The isolated strains were coagulase positive but a variable coagulase activity against the plasma of human, sheep and rabbit. Human plasma gave better results than rabbit and sheep plasma. brinolytic activity of Stapylococcus aureus was tested in oxalated plasma of human, sheep and rabbit. Strains isolated from human surgical wounds were found to be more fibrinolytic as compared to horse isolates. Human and rabbit plasma were a better indicators for checking fibrinolytic activity. None of the isolated strains showed fibrinolytic activity against sheep plasma.
The isolates of Stphlococcus aureus were cultured for alpha haernolysin production in nutrient broth culture. Alpha toxin was purified and its haemolysin activity was determined qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Maximum haemolytic activity was observed against rabbit erythrocytes. Out of hundred strains, sixty-eight produced alpha haemolysin including 45 from human and 23 from horse wounds.
The isolates of Staphylococcus aureus were cultured in semi-solid Heart infusion agar for the production of eta haemolysin. This toxin was purified and its haemolytic activity was determined qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Naximum haemolytic activity was observed against sheep erythrocytes. Out of the hundred strains, beta haemolysin was produced by sixty-two strains including 15 from human and 47 from horses.
The isolates of Staphylococcus aureus were cultured in Brain Heart infusion agar for .the production of delta toxin. This toxin was purified and its haernolytic activity was observed qualitatively and quantitatively against human, sheep and rabbit erythrocytes. Maximum haemolytic activity of dlta toxin was observed against human erythrocytes. Out of the hundred strains, seventy-one produced delta toxin including 43 from human and 28 from horses.
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