Studies On H.S.Oil Adjuvant Vaccine Prepared By Dense Culture Of Pasturella Multocida Robert Type-1 On Improved Culture Medium
By: Kausar Tasneem
| Muhammed Amin Sheikh.
Contributor(s): Manzoor | Muhammed Naeem | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1993Subject(s): Department of MicrobiologyDDC classification: 0304,T Dissertation note: The present project was designed to develop HS oil adjuvant vaccine (CAy) on a special medium. A dense culture was prepared by enriching the routine nutrient broth medium, used for the production of alum-precipitated vaccine (APv) presently. It was concluded that various ingredients, including yeast extract, sucrose, trypticase, sodium bicarbonate, used in proper concentrations, certainly increase the number of bacterial population in the culture medium and help in attaining the requisite 2 mg dry weight of Pasteurella mnultocicla per dose of vaccine. Moreover the organism develops its full antigenic characteristics due to supply of these essentially required nutrients. Aeration of the culture medium is also essential for attaining the dense culture of the organism. Slight agitation was used to provide aeration to the bacteria. The emulsified vaccine was prepared with 15:10:1.5 parts of the bacterial suspension,liquid paraffin and lanolin. The emulsion on prepration, developed a white colour and adhered to clean glass surface evenly. The product proved to possess a long shelf life when maintained at room temperature. Mouse model was chosen to study the safety and potency of both the vaccines i.e. OAV and APV. The potency of both the vaccines was compared and calculated by a standard method of OSE and Muenstar (1968). Oil adjuvant vaccine gave better results of 5.2 log protection as compared to APV, where the log protection value came to 2 only.
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UVAS Library Thesis Section | Veterinary Science | 0304,T (Browse shelf) | Available | 0304,T |
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The present project was designed to develop HS oil adjuvant vaccine (CAy) on a special medium. A dense culture was prepared by enriching the routine nutrient broth medium, used for the production of alum-precipitated vaccine (APv) presently. It was concluded that various ingredients, including yeast extract, sucrose, trypticase, sodium bicarbonate, used in proper concentrations, certainly increase the number of bacterial population in the culture medium and help in attaining the requisite 2 mg dry weight of Pasteurella mnultocicla per dose of vaccine. Moreover the organism develops its full antigenic characteristics due to supply of these essentially required nutrients. Aeration of the culture medium is also essential for attaining the dense culture of the organism. Slight agitation was used to provide aeration to the bacteria. The emulsified vaccine was prepared with 15:10:1.5 parts of the bacterial suspension,liquid paraffin and lanolin. The emulsion on prepration, developed a white colour and adhered to clean glass surface evenly. The product proved to possess a long shelf life when maintained at room temperature. Mouse model was chosen to study the safety and potency of both the vaccines i.e. OAV and APV. The potency of both the vaccines was compared and calculated by a standard method of OSE and Muenstar (1968). Oil adjuvant vaccine gave better results of 5.2 log protection as compared to APV, where the log protection value came to 2 only.
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