To determine the mineral status of indigenous cattle about 300 blood samples were collected from Lahore, Gujranwala, Sheikhupura, Sahiwal, Okara, Faisalabad, Sargodha, Jhang and Gujrat districts of Punjab, and Hyderabad, Mirpurkhas and Karachi districts of Sindh because of having high population of cattle. The blood samples were collected from four different groups of cattle (1) calves (2) heifers (3) dry pregnant (4) lactating non pregnant. The serum was separated from blood samles by centrifugation. Serum sample were analysed for Calcium, Phosphorus, Magnesium, Sodium, Potassium, Zinc, Copper, Manganese, Iron and Cobalt. (Calcium, Phosphorus and Magnesium were analysed on spectronic 20. Sod)1I1 and Potassium were analysed on flamphotometer. Zinc, Copper, Manganese, Iron and Cobalt were analysed on atomic absorption spectrophotometer)The data thus obtained was subjected to statistical analysis by using complete randomized design and the differences in means were tested by using (LSD Test) least significant difference test.

The mean values of serum mineral in cattle of Punjab were, Calcium, 9.34+ 0.41 mg/100 ml, Phosphorus 4.660.25 mg/100 ml, Magnesium 2.02±0.23 mg/100 ml, Sodium 290.61 11.8 mg/l00 ml, Potassium 18.55±0.56 mg/100 ml, Zinc l.06±0.09 ppm, Copper 1.05±0.14 ppm, Manganese .56±0.04 ppm, Iron 1.38±0.06 ppm and Cobalt 0.089±0.007 ppm. The mean value of serum mineral in cattle of Sindh were Calcium 8.83±0.43 mg/100 ml, Phosphorus 4.48±0.23 mg/l00 ml, Magnesium 2.08+0.21 mg/100 ml, Sodium 273.43±18.69 mg/100 ml, Potassium 18.21±0.72 mg/100 ml, Zinc 0.96+0.12 ppm Copper 0.96±0.11 ppm, Manganese 0.54±0.05 ppm, Iron 1.2±0.157 ppm and Cobalt 0.085.±0.009 ppm. Nonsignificant difference in values of serum minerals in cattle (P> 0.05) were found within different groups and between provinces, while significant differences were found between districts (P< 0.05). This variation may be due to different plan of nutrition composition of soil water and climate etc.

The serum Calcium values were almost normal but mild deficiency was found in cattle of Gujrat, Gujranwala, Sheikhupura, Jhang districts of Punjab while Karachi and Mirpurkhas districts of Sindh. The serum Phosphorus level was low in cattle of Punjab and Sindh and acute deficiency was observed in Faisalabad, Gujrat, Shéikhupura and Jhang districts of Punjab, Karachi and Mirpurkhas districts of Sindh. The serum Magnesium values showed deficiency in Sheikhupura, Sargodha and Jhang districts of Punjab and Karachi district of Sindh. The serum Sodium values were almost normal in cattle of all districts. The serum Potassium values were normal in cattle of all districts except Sargodha in Punjab. The serum Zinc values were low in animals of all districts and severe deficiency was found in cattle of Okara, Sahiwal and Sheikhupura districts of Punjab and Karachi Hyderabad, and Mirpurkhas districts of Sindh. The serum Copper values of animals were found low in Okara, Lahore and Sheikhupura districts of Punjab and Karachi and Mirpurkhas districts of Sindh. The serum Manganese values were found deficient in districts of Gujranwala, Gujrat, Sahiwal, Sheikhupura and Jhang in Punjab and Hyderabad district of Sindh. The serum Iron values were normal in animals of both provinces of Punjab and Sindh except Lahore,, Sargodha, Jhang and Sheikhupura districts of Punjab and Hyderabad district in Sindh. The serum Cobalt values were found low in cattle of Sahiwal, Okara and Sheikhupura districts of Punjab and Karachi and Mirpurkhas districts of Sindh. The overall mineral status of cattle of Punjab province was slightly higher than Sindh except Magnesium mineral and the difference was nonsignificant while between the groups of animals were found non significiant. The variation in serum mineral values in cattle might be due to plan nutrition, low mineral status of soil, composition of water and climate etc.