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Studies On Avian Pasteurolosis, Characterization Of Isolates From Diseased Carrier Birds

By: Tanveer Afzal | Sheikh Muhammed Amin.
Contributor(s): S Ata-ur-Rehman Rizvi | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 1992Subject(s): Department of MicrobiologyDDC classification: 0325,T Dissertation note: A total of 1000 birds (600 diseased/dead and 400 healthy) were examined for the isolation of the Pasteurelia muitocida so as to have an idea about the incidence of fowl cholera in the poultry flocks (Broiler and Layer) and the percentage of the carriers for the organism maintiined in and around Lahore. Out of 600 diseased/dead birds examined 1% yielded Pasteurella inultocida. None of the 400 healthy birds proved carrier for organism. All the six isolates produced small, greyish, circular colonies on blood agar. The growth in the Triptic Soya agar appeared in the form of convex, entire edged, mucoid colonies having a diameter of ljim. There was not any growth on MacConkey agar. All the isolates fermented glucose, sucrose, mannose, mannitol, xylose and fructose with production of both, acid and gas. Likewise all the six isolates were positive for nitrate reduction, catalase/reduction, indole production H2sproduction nitrate reduction tests. None of the isolates was positive for gelatin liquification methyl red test, voges-Proskauer test, and urease production test. All the isolated organisms were non-motile. All the isolates proved pathogenic to mice. Antibitics including chloramphenicol, Gentamicin Furadantin, Streptomycin and Nalidixic acid were highly effective against all the isolated strains. However the isolates were insensitive to Tetracycline and Erythromycin.
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Veterinary Science 0325,T (Browse shelf) Available 0325,T
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A total of 1000 birds (600 diseased/dead and 400 healthy) were examined for the isolation of the Pasteurelia muitocida so as to have an idea about the incidence of fowl cholera in the poultry flocks (Broiler and Layer) and the percentage of the carriers for the organism maintiined in and around Lahore.

Out of 600 diseased/dead birds examined 1% yielded Pasteurella inultocida. None of the 400 healthy birds proved carrier for organism. All the six isolates produced small, greyish, circular colonies on blood agar. The growth in the Triptic Soya agar appeared in the form of convex, entire edged, mucoid colonies having a diameter of ljim.

There was not any growth on MacConkey agar. All the isolates fermented glucose, sucrose, mannose, mannitol, xylose and fructose with production of both, acid and gas. Likewise all the six isolates were positive for nitrate reduction, catalase/reduction, indole production H2sproduction nitrate reduction tests.

None of the isolates was positive for gelatin liquification methyl red test, voges-Proskauer test, and urease production test. All the isolated organisms were non-motile.

All the isolates proved pathogenic to mice. Antibitics including chloramphenicol, Gentamicin Furadantin, Streptomycin and Nalidixic acid were highly effective against all the isolated strains. However the isolates were insensitive to Tetracycline and Erythromycin.

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