Effect Of Milk Fever On Serum Calcium, Phosphorus And L.D.H. In Cattle And Buffaloes
By: Saeed, M | Muhammed sarwer Khan.
Contributor(s): Muhammed Younas Chaudry | Faculty of Veterinary Sciences.
Material type: BookPublisher: 1992Subject(s): Department of Clinical Medicine & SurgeryDDC classification: 0330,T Dissertation note: In this study affect of milk fever was investigated on serum creatine phospholinase (C .P .K.) and lactic dehdrogenase (L .D .H). The experiments were performed on 50 cows/buffaloes affect with this disease. 50 healthy animals were also investigated as a control group. Blood samples were collected directly from the diseased animals. Serum was separated from the blood by the method of centrifugation at the rate of 3000 r.p.m - The serum samples were stored at -20oC in the freezer. The analysis were done by the help of special kits and spectrophotometer. This whole work of analysis was performed in the medicine laboratory at college of veterinary Sciences Lahore. The results of diseased and healthy animals were calculated. This research shows that during the milk fever the calcium level becomes low than the normal. The serum creatine phosphokinase (C.P.K) and Lactic dehydrogenase (L.D.H) are two body enzymes. The activity of these enzymes was elevated than the normal. The mean value of the calcium in 50 diseased animals was 4.90 mg/dl (Which is low than normal). the mean value of the serum enzyme creatine phosphokinase (CP.K) was 306.54 u/i (Which is high than the normal) . The mean value of serum enzyme lactic dehydrogenase (L.D.H) was 1890.42 (which is high than normal. It was investigated to see the effect of hypocalcemia on two body enzymes the C.P.K and L.D.H. At the end of the study of this research the laboratory data shows that ultimately thre is an elevation in the activities of the said enzymes. Which is a great investigation for the diagnostic data of the milk fever.Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis | UVAS Library Thesis Section | Veterinary Science | 0330,T (Browse shelf) | Available | 0330,T |
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In this study affect of milk fever was investigated on serum creatine phospholinase (C .P .K.) and lactic dehdrogenase (L .D .H). The experiments were performed on 50 cows/buffaloes affect with this disease. 50 healthy animals were also investigated as a control group. Blood samples were collected directly from the diseased animals. Serum was separated from the blood by the method of centrifugation at the rate of 3000 r.p.m - The serum samples were stored at -20oC in the freezer. The analysis were done by the help of special kits and spectrophotometer. This whole work of analysis was performed in the medicine laboratory at college of veterinary Sciences Lahore. The results of diseased and healthy animals were calculated.
This research shows that during the milk fever the calcium level becomes low than the normal. The serum creatine phosphokinase (C.P.K) and Lactic dehydrogenase (L.D.H) are two body enzymes. The activity of these enzymes was elevated than the normal. The mean value of the calcium in 50 diseased animals was 4.90 mg/dl (Which is low than normal). the mean value of the serum enzyme creatine phosphokinase (CP.K) was 306.54 u/i (Which is high than the normal) . The mean value of serum enzyme lactic dehydrogenase (L.D.H) was 1890.42 (which is high than normal.
It was investigated to see the effect of hypocalcemia on two body enzymes the C.P.K and L.D.H. At the end of the study of this research the laboratory data shows that ultimately thre is an elevation in the activities of the said enzymes. Which is a great investigation for the diagnostic data of the milk fever.
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