Factors Affecting Haemagglutination Activity Of Newcastle Disease Virus
By: Monem Rizvi, Syed
| Dr. Muhammad Akram Munir.
Contributor(s): Dr. Mian Mubasher Naeem | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1993Subject(s): Department of MicrobiologyDDC classification: 0353,T Dissertation note: While standardizing various components of haemagglutination (HA) test optimum results were obtained using phosphate buffer saline (PBS) as a diluent, 0.5 per cent red blood cells suspended in PBS as indicator, and by incubating the microtitration plates at 4C. While best Haemagglutination-Inhibition (HI) titers were obtained by using 4 HA Units of antigen and 60 minutes incubation period (time given for antigen-serum reaction). The HI test did not detect maternal antibodies in the serum of birds after 4 week of age. Initial vaccination of chicks at 7 days of age followed by a booster dose at 21 days of age, elicited a good immune response and protected the broilers against virulent NDV challenge at 49 days of age. Administration of a single dose of NDV vaccine at 7 day of age or three NDV vaccine doses, one for priming at 7 days of age, second for first boosting at 21 days of age and third as a second booster dose at 35 days of age were not recommended, as in first case the immunity was short lived and in second case birds were exposed to unnecessary stress.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0353,T (Browse shelf) | Available | 0353,T |
While standardizing various components of haemagglutination (HA) test optimum results were obtained using phosphate buffer saline (PBS) as a diluent, 0.5 per cent red blood cells suspended in PBS as indicator, and by incubating the microtitration plates at 4C. While best Haemagglutination-Inhibition (HI) titers were obtained by using 4 HA Units of antigen and 60 minutes incubation period (time given for antigen-serum reaction). The HI test did not detect maternal antibodies in the serum of birds after 4 week of age. Initial vaccination of chicks at 7 days of age followed by a booster dose at 21 days of age, elicited a good immune response and protected the broilers against virulent NDV challenge at 49 days of age. Administration of a single dose of NDV vaccine at 7 day of age or three NDV vaccine doses, one for priming at 7 days of age, second for first boosting at 21 days of age and third as a second booster dose at 35 days of age were not recommended, as in first case the immunity was short lived and in second case birds were exposed to unnecessary stress.
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