Titration Of Infectious Bursal Disease Virus In Different Organs Of Gumboro Infected Birds And Embryonated Hen
By: Saeed Anwar, M
| Dr. Muhammad Naeem.
Contributor(s): Dr. Haji Ahmad | Dr. Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1995Subject(s): Department of MicrobiologyDDC classification: 0458,T Dissertation note: Infectious bursal disease virus (IBDV) was quantitated in different organs of the infected birds and in different parts of the experimentally inoculated embryonated hen eggs through agar gel precipitation technique (AGPT). The AGPT was standardized in the laboratory using known hyperimmune sera against IBDV antigen. The concentration of the agar, antibody and antigen are important factors for the development of precipitation line. Moreover, only gel containing noble agar in distilled water is important for diffusion of the antigen/antibody, while either of the other reagents such as sodium chloride, phenol crystal and sodium azide are presumably added to inhibit the microbial growth.
The serum from birds on 14 days post first bost showed 294.1 ACPT titers, while egg yolk from these bird on 30-45 days post first boost were having 68.6 AGPT titers. Postmortem examination showed the lesions
In liver,Kidney, spleen, intestine and bursa fabricius. It was observed that only bursa of fabricius showed detectable level (1:4 to 1:16) of IBD virus titers. Similarly, chorioaflantoic membrane of the inoculated egg showed 1:4 AGPT titers. From this study it was concluded that AGPT is a crude but highly reliable test for qualitative and quantitative study of IBDV antigen or antibodies.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0458,T (Browse shelf) | Available | 0458,T |
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Infectious bursal disease virus (IBDV) was quantitated in different organs of the infected birds and in different parts of the experimentally inoculated embryonated hen eggs through agar gel precipitation technique (AGPT). The AGPT was standardized in the laboratory using known hyperimmune sera against IBDV antigen. The concentration of the agar, antibody and antigen are important factors for the development of precipitation line. Moreover, only gel containing noble agar in distilled water is important for diffusion of the antigen/antibody, while either of the other reagents such as sodium chloride, phenol crystal and sodium azide are presumably added to inhibit the microbial growth.
The serum from birds on 14 days post first bost showed 294.1 ACPT titers, while egg yolk from these bird on 30-45 days post first boost were having 68.6 AGPT titers. Postmortem examination showed the lesions
In liver,Kidney, spleen, intestine and bursa fabricius. It was observed that only bursa of fabricius showed detectable level (1:4 to 1:16) of IBD virus titers. Similarly, chorioaflantoic membrane of the inoculated egg showed 1:4 AGPT titers. From this study it was concluded that AGPT is a crude but highly reliable test for qualitative and quantitative study of IBDV antigen or antibodies.
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