Changes In The Harderian Gland And Respiratory Tract Of The Broiler Chicken Following Intraocular Vaccination And Infection With/Against Newcastle Disease Virus
By: Amer Jumal, M
| Dr. Shakil Akhtar Khan.
Contributor(s): Dr. Ahmad Raza | Dr. M. Asif Rabbani | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1997Subject(s): Department of PathologyDDC classification: 0504,T Dissertation note: This project was designed to determine the immune response, macroscopic and microscopic changes in the Harderian gland (HG) and respiratory organs of young chickens reared in well controlled environment following eye-drop vaccination against ND and subsequent challenge with a NDV field strain. This endeavour also represents an effort to raise the curtain on the extent of local and humoral immunity in response to eye-drop vaccination against NDV and also after challenge by two different routes (intraocular and intratracheal).For this project a total of 150 day old broiler chicks were procured from local commercial hatchery. At day one 6 birds were used to determine the maternal antibody titres against ND. On day 7, chicks were randomly subdivided into 6 subgroups Al and A2, Bi and B2, Cl and C2, each subgroup having 24 birds. Three subgroups were vaccinated through eye-dropping against NDV (i.e. Al, A2 and Cl) at 7th and 21st day of age. The other three sub-groups were kept non-vaccinated (i.e. Bi, B2 and C2). Challenge of virulent NDV was administered at 28t1i day of age by two different routes to the different subgroups. Intraocular challenge was given to Al and Bi subgroups and intratracheal challenge was given to A2 and B2 subgroups, whereas Cl and C2 subgroups were kept as control. Determination of antibody response against NDV by heamagglutination inhibition test and gross and histopathology of Harderian glands, trachea and lungs of six birds of all experimental and control subgroups were performed on days 14, 28, 31 and 35 of age. Ocular vaccination engendered higher level of circulating antibodies against NDV but developed poor local protective immunity in tracheal rrucosa. Intratracheal challenge, caused pronounced changes in vaccinateçl birds by eye-dropping in comparison to intraocular challenge, so it can be infered that route of challenge of NDV played a characteristic' role in developing disease to the host vaccinated through eye-droppings.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0504,T (Browse shelf) | Available | 0504,T |
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This project was designed to determine the immune response, macroscopic and microscopic changes in the Harderian gland (HG) and respiratory organs of young chickens reared in well controlled environment following eye-drop vaccination against ND and subsequent challenge with a NDV field strain. This endeavour also represents an effort to raise the curtain on the extent of local and humoral immunity in response to eye-drop vaccination against NDV and also after challenge by two different routes (intraocular and intratracheal).For this project a total of 150 day old broiler chicks were procured from local commercial hatchery. At day one 6 birds were used to determine the maternal antibody titres against ND. On day 7, chicks were randomly subdivided into 6 subgroups Al and A2, Bi and B2, Cl and C2, each subgroup having 24 birds. Three subgroups were vaccinated through eye-dropping against NDV (i.e. Al, A2 and Cl) at 7th and 21st day of age. The other three sub-groups were kept non-vaccinated (i.e. Bi, B2 and C2). Challenge of virulent NDV was administered at 28t1i day of age by two different routes to the different subgroups. Intraocular challenge was given to Al and Bi subgroups and intratracheal challenge was given to A2 and B2 subgroups, whereas Cl and C2 subgroups were kept as control. Determination of antibody response against NDV by heamagglutination inhibition test and gross and histopathology of Harderian glands, trachea and lungs of six birds of all experimental and control subgroups were performed on days 14, 28, 31 and 35 of age. Ocular vaccination engendered higher level of circulating antibodies against NDV but developed poor local protective immunity in tracheal rrucosa. Intratracheal challenge, caused pronounced changes in vaccinateçl birds by eye-dropping in comparison to intraocular challenge, so it can be infered that route of challenge of NDV played a characteristic' role in developing disease to the host vaccinated through eye-droppings.
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