The effect of dietary aflatoxin and detoxified aflatoxin was studied on postmortem lesion, total serum protein, serum bilirubin, SGOT and SGPT in commercial broiler, which were fed on broiler starter ration upto 21 days. From 21st day to 42nd day they were divided into 5 group and fed toxicated feed along with different treatment. Aflatoxins were produced on corn by moistened it at room temperature for 15 days, and the concentration of aflatoxin in corn was determined through direct ELISA (Brinton, 1987) from a commercial Laboratory. This corn were ground and appropriate amount of this was mixed into broiler finisher ration to generate 70 ppb aflatoxin B1 level.

One hundred day old broiler chicks were obtained from a commercial hatchery and fed on broiler starter ration upto 21 days. At 21st day these were randomly divided into 5 groups, having 20 birds each. Each birds in each. One group was received simple feed, 2nd group was received contaminated feed with aflatoxin B1 70 ppb, 3rd, 4th, and 5th group were received contaminated feed with 70 ppb aflatoxin and treated for detoxification with extrusion, sodium bentonite and Mycofix plus inclusion respectively.

All the birds were thoroughly observed throughout the experimental period to detect any clinical sign deviated from normal. All the birds of each group were slaughtered at the end of experiment at 42nd days their blood was taken for separation of serum and gross postmortem lesions were recorded.

The common clinical symptoms observed in chicken at 70 ppb level were dullness, depression, paleness of combs, wattles and shank. The severity of these symptoms were less in groups feed a diet contaminated with aflatoxin with detoxification with extrusion and Mycofix plus. The group fed diet containing sodium bentonite, normal combs, wattles and shanks. Birds were remained active.

The postmortem changes in the group receiving 70 ppb dietary aflatoxin showed the enlargement of liver, kidney, pancreas and spleen. Haemorrhages were present on pancreas and on intestine. The severity of lesion on these organs were less in groups fed on diet contaminated with aflatoxin with detoxification with extrusion, Mycofix plus and sodium bentonite. The group fed on sodium bentonite added contaminated diet has normal intestine.

The total serum protein in group 2 decreased at 70 ppb aflatoxin level in feed. In group 3 which was treated with extrusion serum protein level also decreased. In group which was treated with sodium bentonite protein level increased and showed much improvement. In group 5 it also increased but less than group 4. Increase was highly significant (P<0.01) at the end of experiment.

The total serum bilirubin in group 2 increased at 70 ppb aflatoxin level. Improvement was in group 3,5 and 4 which were treated with extrusion, Mycofix plus and sodium bentonite. Much improvement in group 3 whose feed was detoxified with extrusion process. Decrease was highly significant (P<0.01) at the end of experiment.

The activity of SGOT in group 2 were increased at 70 ppb aflatoxin level in feed as compared to control. The activity of SGOT in treated groups were also increased. The treatment means of SGOT differed significantly (P<0.01).

The activity of SGPT in group 2 was increased at 70 ppb aflatoxin level in feed as compared to control. The activity of SGPT is treated group were also increased. The treatment means of SGPT differed significantly (P<0.01).

It was concluded from this study that the aflatoxin contaminated feed can be detoxified with extrusion, Mycofix plus or with addition of sodium bentonite. Addition of these in the feed improved the general body condition and liver function of the chickens.