An investigation was undertaken to study the protective effect of indomethacin and powdered Cuminum nigrum Linn. Seeds (Kala zeera) on alloxan induced diabetes in rabbits.

Thirty healthy rabbits were kept in animal house of College of Veterinary Sciences, Lahore. These rabbits were randomly divided into six groups A, B, C, D, E and F comprising of 5 rabbits in each and were provided food and water aci-libitum.

Group A was kept as control which was given alloxan (150mg/Kg body weight) without prior administration of indomethacin or Kala zeera. Group B rabbits were given 10 mg/Kg body weight indornethacin intraperitoneally, 15 minute prior to alloxan administraiton intravenously of 150mg/Kg body weight. Group C was given 20mg/Kg body weight of indomethacin intraperitoneally 15 minute prior to alloxan administration intravenously of 150mg/Kg body weight. Group D rabbits were given powdered Cuminum nigrurn 2gm/Kg body weight orally 2 hours prior to alloxan administraiton intravenously of 150mg/Kg body weight. Group E was given only 10mg/Kg body weight of indomethacin intrperitoneally and its effect was noted. Group F was given only 25mg i.e. 0.5m1 NaHCO3 5% intraperitoneally to see its effect.

The animals were sampled for glucose and protein estimation after zero 24, 48 and 72 hours. Before taking the samples animals were fasted overnight. Their blood glucose levels were estimated by glucorneter and total serum protein was estimated by Biuret method. Group A showed marked increase of blood glucose level and had not effect on total protein. Group B did not show protective effect on blood glucose level and no change in total serum protein level, while group C showed significantly protective effect on the blood glucose level and no change in total serum protein level. Group D showed marked increase of blood glucose level and did not show any protective effect and also no change in total serum protein level. Group E and F showed significant increase of blood glucose level but increased level remained in the normal limit and no change in total serum protein.

It is clear that indomethacin showed dose dependently protective effect on alloxan induced diabetes in rabbits but herbal medicine Cuminum nigrum has no protective effect. It is also clear that there in no change in total serum protein level even in severe diabetic condition.