Standardization Of Indirect Haemagglutination Test For Surveillance Of Antibodies To Mycoplasma Capri
By: Shaukat Ali
| Dr.Muhammad Amin Sheikh.
Contributor(s): Dr.Asif Rabbani | Dr.Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1999Subject(s): Department of MicrobiologyDDC classification: 0637,T Dissertation note: The present study was carried out to standardize indirect haemagglutination test for surveillance of antibodies against Mycoplasma capri. The Mvconlasma mvcoides sub species capri strain PG3, procured from Veterinary Research Institute, Quetta was used as antigen in the test. The strain was processed for its optimal growth in brain heart infusion broth and subsequently developed into an oil based vaccine for raising hyperimmune serum in the rabbit. The titre of the latter was made a base for knowing influence of various factors to be studied.
The factors examined included the antigen dilution to sensitize erythrocytes, nature and concentration of coupling agent (Tannic acid, glutaraldehyde), source of erythrocytes, interaction time and temperature.
One percent sheep and human group 'O' erythrocytes sensitized with antigeh through tannic acid concentration of 0.5% in phosphate buffer saline at 37oC for 10 minutes gave a titre respectively of 1:64 and 1:16.
None of the tannic acid concerntrations used (0.5, 0.05, 0.005%) sensitized poultry erythrocytes with antigen at either time interaction.
All the various glutaradehyde concentrations used (0.1, 0.2, 0.25, 0.5%) were unable to sensitize erythrocytes, with antigen, irrespective of the source concerned i.e. sheep, human or poultry sources.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0637,T (Browse shelf) | Available | 0637,T |
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The present study was carried out to standardize indirect haemagglutination test for surveillance of antibodies against Mycoplasma capri. The Mvconlasma mvcoides sub species capri strain PG3, procured from Veterinary Research Institute, Quetta was used as antigen in the test. The strain was processed for its optimal growth in brain heart infusion broth and subsequently developed into an oil based vaccine for raising hyperimmune serum in the rabbit. The titre of the latter was made a base for knowing influence of various factors to be studied.
The factors examined included the antigen dilution to sensitize erythrocytes, nature and concentration of coupling agent (Tannic acid, glutaraldehyde), source of erythrocytes, interaction time and temperature.
One percent sheep and human group 'O' erythrocytes sensitized with antigeh through tannic acid concentration of 0.5% in phosphate buffer saline at 37oC for 10 minutes gave a titre respectively of 1:64 and 1:16.
None of the tannic acid concerntrations used (0.5, 0.05, 0.005%) sensitized poultry erythrocytes with antigen at either time interaction.
All the various glutaradehyde concentrations used (0.1, 0.2, 0.25, 0.5%) were unable to sensitize erythrocytes, with antigen, irrespective of the source concerned i.e. sheep, human or poultry sources.
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