Comparative Sensitivity Of Agpt, Iha & Elisa In Determing The Antibody Response Against Ibd Virus Using Intermediate Plus (Hot) Strain 228E and Bur706
By: Asim, M
| Dr . Muhammad Naeem.
Contributor(s): Dr . Khalid | Dr . Muhammad Akram Munir | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1999Subject(s): Department of MicrobiologyDDC classification: 0675,T Dissertation note: The present project was aimed at determining the comparative sensitivity of AGPT, IHA and ELISA. By determining the maternal antibody titre against IBDV. The protective efficacy of vaccines 228-E, BUR-706 and Gumboral CT was also evaluated by challenging the vaccinated birds with virulent IBD virus. A total of 200 day-old birds were randomly divided into four equal groups designated A, B, C and D. Chicks of group A, B and C were vaccinated with 228-F, BUR-706 and Gumboral CT, respectively. Group B served as un-vaccinated control.
ELISA was found more sensitive than AGPT and IHA in determining maternal antibody titre. Higher antibody titres were produced by 228-F compared to BUR-706 and Gumboral CT, respectively. Maximum mean ELISA titres in group A, B, C and D on day 35 were 10,080, 8539, 7022 and 537, respectively.
Decline in maternal antibody titre was observed weekly. AGPT could detect it up to day 14, while IHA up to day 2] and ELISA up to day 35.
228-F [Intermediate Hot (Plus) strain] provided maximum protection in vaccinated birds when challenged on day 20 and day 35 post-vaccination.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0675,T (Browse shelf) | Available | 0675,T |
The present project was aimed at determining the comparative sensitivity of AGPT, IHA and ELISA. By determining the maternal antibody titre against IBDV. The protective efficacy of vaccines 228-E, BUR-706 and Gumboral CT was also evaluated by challenging the vaccinated birds with virulent IBD virus. A total of 200 day-old birds were randomly divided into four equal groups designated A, B, C and D. Chicks of group A, B and C were vaccinated with 228-F, BUR-706 and Gumboral CT, respectively. Group B served as un-vaccinated control.
ELISA was found more sensitive than AGPT and IHA in determining maternal antibody titre. Higher antibody titres were produced by 228-F compared to BUR-706 and Gumboral CT, respectively. Maximum mean ELISA titres in group A, B, C and D on day 35 were 10,080, 8539, 7022 and 537, respectively.
Decline in maternal antibody titre was observed weekly. AGPT could detect it up to day 14, while IHA up to day 2] and ELISA up to day 35.
228-F [Intermediate Hot (Plus) strain] provided maximum protection in vaccinated birds when challenged on day 20 and day 35 post-vaccination.
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