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A Study On Physico Chemical Actors Affecting The Survial Of Avian Influenza (H9 N2) Virus

By: Tehmina Sadaf | Dr . Sameera Akhtar.
Contributor(s): Dr . Khushi Muhammad | Dr . Shakil | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 2001Subject(s): Department of MicrobiologyDDC classification: 0703,T Dissertation note: A total of 310 chicken embryos (9 day old) were purchased from local market. The embryos were incubated at 37°C. On eleventh day of age avian influenza virus (H9 N2) for its propagation was inoculated in 10 chicken embryos. The four haemagglutination titer (4HA) of the virus was prepared to observe the response to various physical and chemical factors. Physical factors included were temperature, pH and UV (280 nm) light. The virus, exposed to the physical factors for different time intervals was inoculated into embryos through allantoic route. These embryos were kept in an incubator (37°C) for 72 hours. Later on the allantoic amniotic fluid (AAF) from each inoculated embryos subjected to spot haemagglutination test. The virus endured 56 °C temperature for 15 and 30 minutes while got inactivated in 45 minutes. More over virus survived at pH 7 for 15, 30 and 45 minutes but lost its HA activity at pH 5 and 9 in 15, 30 and 45 minutes. It was further examined that virus survived after 60 minutes exposure UV light but inactivated after 90 minutes. The disinfectants formalin, phenol, iodine solution and fin virus were used in 0.5%, 1% and 1.5% concentrations. The 4HA titer of virus was mixed in various concentrations of chemical disinfectants and was inoculated into embryonated eggs. The AAF of these eggs was subjected to spot agglutination test. The results of the test showed that all four chemicals formalin, phenol, iodine and fin virus inactivated the virus in 0.5% concentration in 15 minutes and all have good antiviral activity against avian influenza virus.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
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Veterinary Science 0703,T (Browse shelf) Available 0703,T
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A total of 310 chicken embryos (9 day old) were purchased from local market. The embryos were incubated at 37°C. On eleventh day of age avian influenza virus (H9 N2) for its propagation was inoculated in 10 chicken embryos. The four haemagglutination titer (4HA) of the virus was prepared to observe the response to various physical and chemical factors. Physical factors included were temperature, pH and UV (280 nm) light. The virus, exposed to the physical factors for different time intervals was inoculated into embryos through allantoic route. These embryos were kept in an incubator (37°C) for 72 hours. Later on the allantoic amniotic fluid (AAF) from each inoculated embryos subjected to spot haemagglutination test. The virus endured 56 °C temperature for 15 and 30 minutes while got inactivated in 45 minutes. More over virus survived at pH 7 for 15, 30 and 45 minutes but lost its HA activity at pH 5 and 9 in 15, 30 and 45 minutes. It was further examined that virus survived after 60 minutes exposure UV light but inactivated after 90 minutes. The disinfectants formalin, phenol, iodine solution and fin virus were used in 0.5%, 1% and 1.5% concentrations. The 4HA titer of virus was mixed in various concentrations of chemical disinfectants and was inoculated into embryonated eggs. The AAF of these eggs was subjected to spot agglutination test. The results of the test showed that all four chemicals formalin, phenol, iodine and fin virus inactivated the virus in 0.5% concentration in 15 minutes and all have good antiviral activity against avian influenza virus.

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