Comparative Immunopathological And Immunosuppressive Effects Of Three Different Gumboro Vacine Strains
By: Rana Muhammad Ayyub
| Dr. Asim Aslam.
Contributor(s): Dr. Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2002Subject(s): Department of PathologyDDC classification: 0775,T Dissertation note: This project was planned to know the comparative immunosuppressive effects of three different Gumboro live vaccine strains on ND vaccination and to choose the least immunosuppressive out of these three vaccine strains.
A total of 100 chicks were divided into 4 groups (A, B, C and D), 25 birds in each group. All the birds of all the groups were vaccinated against Newcastle disease on 5th and 21st day of age. Specific IBD vaccine was given to specific groups at l4t1 day and 28th day of age. Group A was given intermediate plus strain vaccine (228 E strain), group B was given intermediate strain vaccine (Strain D78) and group C was given another intermediate strain vaccine (Bursine-2 strain), on both days of IBD vaccination, as per schedule. While group D was unvaccinated control.
Immune organs including bursa, thymus and spleen were examined for their gross and microscopic pathological changes, before and after IBD vaccination. For this purpose, these organs were collected at 13th j7th and 31st days of age. At j3th day, gross and histopathological examination showed no lesions in any case of any group (as so far no IBD vaccine was given ). At 17th and 31st days, gross and histopathological examination revealed more severe lesions in group A, moderate lesions in group B, mild lesions in group C and no lesion in immune organs of group D.
Haemagglutination inhibition (HI) test was performed, to study the comparative humoral immunosuppressive effects of these IBD vaccines on antibody titre against ND vaccination, on birds of each group. Statistical analysis showed that 228 E vaccine strain (group A) while, D78 vaccine strain (group B) was less immunosuppressive but, on the other hand Bursine-2 vaccine strain (group C) was least immunosuppressive. No humoral immunosuppression was observed in unvaccinated control group D.
This study suggests the use of Bursine2 strain of IBD vaccine, in a flock at risk of ND infection, as it has least immunosuppressive effect against ND vaccination. Therefore, still the need of the hour is to choose the most proper IBD vaccine strain, among all the available strains in the market, which can provide excellent protection against IBD infection as well as has least immunosuppressive effects on ND vaccination.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0775,T (Browse shelf) | Available | 0775,T |
This project was planned to know the comparative immunosuppressive effects of three different Gumboro live vaccine strains on ND vaccination and to choose the least immunosuppressive out of these three vaccine strains.
A total of 100 chicks were divided into 4 groups (A, B, C and D), 25 birds in each group. All the birds of all the groups were vaccinated against Newcastle disease on 5th and 21st day of age. Specific IBD vaccine was given to specific groups at l4t1 day and 28th day of age. Group A was given intermediate plus strain vaccine (228 E strain), group B was given intermediate strain vaccine (Strain D78) and group C was given another intermediate strain vaccine (Bursine-2 strain), on both days of IBD vaccination, as per schedule. While group D was unvaccinated control.
Immune organs including bursa, thymus and spleen were examined for their gross and microscopic pathological changes, before and after IBD vaccination. For this purpose, these organs were collected at 13th j7th and 31st days of age. At j3th day, gross and histopathological examination showed no lesions in any case of any group (as so far no IBD vaccine was given ). At 17th and 31st days, gross and histopathological examination revealed more severe lesions in group A, moderate lesions in group B, mild lesions in group C and no lesion in immune organs of group D.
Haemagglutination inhibition (HI) test was performed, to study the comparative humoral immunosuppressive effects of these IBD vaccines on antibody titre against ND vaccination, on birds of each group. Statistical analysis showed that 228 E vaccine strain (group A) while, D78 vaccine strain (group B) was less immunosuppressive but, on the other hand Bursine-2 vaccine strain (group C) was least immunosuppressive. No humoral immunosuppression was observed in unvaccinated control group D.
This study suggests the use of Bursine2 strain of IBD vaccine, in a flock at risk of ND infection, as it has least immunosuppressive effect against ND vaccination. Therefore, still the need of the hour is to choose the most proper IBD vaccine strain, among all the available strains in the market, which can provide excellent protection against IBD infection as well as has least immunosuppressive effects on ND vaccination.
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