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Passive Immunization Against Hydropericardium Syndrome Infected Broilers

By: Ghazanfar khalid | Dr. Khushi Muhamad.
Contributor(s): Dr. Masood Rabbani | Dr. Shakeel | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 2002Subject(s): Department of MicrobiologyDDC classification: 0781,T Dissertation note: In this project, passive immunization against hydropericardium syndrome virus (HPSV) infected broiler chicks was studied. The hyperimmune yolk and serum were raised in commercial layers by priming and boosting with formaldehyde inactivated HPSV vaccine. It was found that yolk and serum collected from the layers showed high titres of indirect haemagglutination (IHA) antibodies against HPS virus. Lethal dose 50 (LD50) of the HPS virus infected liver homogenate was calculated to be 1O-55/ml. It was noted that the broiler chicks (26 days old) receiving yolk containing 256, 128, 64 and 32 units of IHA-anti-HPSV antibodies and virulent HPSV, simultaneously showed 100%, 100%, 100% and 60% protection. While the broiler chicks receiving serum containing 128, 64, 32 and 16 units of IHA anti-HPSV antibodies and virulent HPSV, simultaneously showed 100%, 100%, 40% and zero protection. The birds receiving yolk and serum of control group showed zero protection. It was observed that egg yolk (lml, 64 IHA-anti HPSV-antibodies) injected 24 hours before, at the same time and after 24 hours to HPSV challenged broilers showed 100% protection. While the clinically healthy birds 48 hours post challenge infection showed 60% protection and birds showing signs of the disease showed 20% protection. The cost of the production of hyperimmune yolk was Rs. 0.50/dose (lml: 64 IHA units of anti-HPSV antibodies). It was concluded that hyperimmune yolk could be used to cure the HPSV infected birds if administered immediately in the affected flocks.
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In this project, passive immunization against hydropericardium syndrome virus (HPSV) infected broiler chicks was studied. The hyperimmune yolk and serum were raised in commercial layers by priming and boosting with formaldehyde inactivated HPSV vaccine. It was found that yolk and serum collected from the layers showed high titres of indirect haemagglutination (IHA) antibodies against HPS virus. Lethal dose 50 (LD50) of the HPS virus infected liver homogenate was calculated to be 1O-55/ml.

It was noted that the broiler chicks (26 days old) receiving yolk containing 256, 128, 64 and 32 units of IHA-anti-HPSV antibodies and virulent HPSV, simultaneously showed 100%, 100%, 100% and 60% protection. While the broiler chicks receiving serum containing 128, 64, 32 and 16 units of IHA anti-HPSV antibodies and virulent HPSV, simultaneously showed 100%, 100%, 40% and zero protection. The birds receiving yolk and serum of control group showed zero protection. It was observed that egg yolk (lml, 64 IHA-anti HPSV-antibodies) injected 24 hours before, at the same time and after 24 hours to HPSV challenged broilers showed 100% protection. While the clinically healthy birds 48 hours post challenge infection showed 60% protection and birds showing signs of the disease showed 20% protection.

The cost of the production of hyperimmune yolk was Rs. 0.50/dose (lml: 64 IHA units of anti-HPSV antibodies).

It was concluded that hyperimmune yolk could be used to cure the HPSV infected birds if administered immediately in the affected flocks.

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