Effect Of Experimental Yolk Sac Infection With Escherichia Coli On Immune Status Of Broiler Chicks
By: Muhammad Salah-ud-Shah
| Dr. Shakil Akhtar Khan.
Contributor(s): Dr. Asim Aslam | Dr. Masood Rabbani | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2002Subject(s): Department of PathologyDDC classification: 0790,T Dissertation note: The present project was designed to evaluate the effect of experimental yolk sac infection with E. coli on immune status of broiler chicks. One hundred day old broiler chicks were divided in to two groups i.e. A and B containing 50 birds each. Experimental infection with E. coli was given intra yolk to group A on day-1 of experiment, while group B was kept as control. Ten chicks from each group were sacrificed to collect serum and yolks on 3rd, 5th, 7th and 9th day. Parameters studied were pathological examination of yolk sac, yolk sac/body weight ratio, antibody titer against Newcastle disease virus and analysis of fractional serum proteins.
Results of this experiment showed that yolk sac infection with E. coli led to gross pathological changes of yolk sac (enlarged, discoloured, abnormal consistency and congested blood vessels) and increased yolk sac/body weight ratio. Geometric mean titer against NDV was decreased in serum and yolk of treatment group. Analysis of fractional serum proteins by SDS-PAGE revealed that percentage area covered by most of serum protein fractions was decreased in treatment group as compared to control group.
It was concluded that intra yolk infection with E. coli resulted in pathological changes of yolk sac, increased yolk sac/body weight ratio and decreased immunity, which was evident from decreased maternal antibody titers and altered serum proteins profile of chicks.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0790,T (Browse shelf) | Available | 0790,T |
The present project was designed to evaluate the effect of experimental yolk sac infection with E. coli on immune status of broiler chicks. One hundred day old broiler chicks were divided in to two groups i.e. A and B containing 50 birds each. Experimental infection with E. coli was given intra yolk to group A on day-1 of experiment, while group B was kept as control. Ten chicks from each group were sacrificed to collect serum and yolks on 3rd, 5th, 7th and 9th day. Parameters studied were pathological examination of yolk sac, yolk sac/body weight ratio, antibody titer against Newcastle disease virus and analysis of fractional serum proteins.
Results of this experiment showed that yolk sac infection with E. coli led to gross pathological changes of yolk sac (enlarged, discoloured, abnormal consistency and congested blood vessels) and increased yolk sac/body weight ratio. Geometric mean titer against NDV was decreased in serum and yolk of treatment group. Analysis of fractional serum proteins by SDS-PAGE revealed that percentage area covered by most of serum protein fractions was decreased in treatment group as compared to control group.
It was concluded that intra yolk infection with E. coli resulted in pathological changes of yolk sac, increased yolk sac/body weight ratio and decreased immunity, which was evident from decreased maternal antibody titers and altered serum proteins profile of chicks.
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