A Study On Bacteriological Examination Of Raw And Pasteurized Milk Marketed In Lahore Area With Particular Reference To Public Health
By: Aman Ullan
| Dr.Muhammad Akram Munir.
Contributor(s): Dr.Khalid Saeed | Dr.Masood Rubani | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2001Subject(s): Department of MicrobiologyDDC classification: 0802,T Dissertation note: The present study was conducted to study the bacteriological quality of various types of milk being marketed in Lahore. A total of 120 milk samples consisting of equal number (30 each of raw, pasteurized, UHT and sterilized milk) from various sources were examined for standard plate counts using milk agar, coliform count using EMB agar and using milk ring test (MRT) for brucellosis.
All the samples were negative for MRT. Average standard plate count (SPC) and coliform counts for raw milk were 2.34x107 cfulml and 2.2x105 cfu/ml respectively. Similarly, SPC and coliform counts for pasteurized milk were found as 2.16x105 cfuJml and 3.28x104 cfu/ml respectively. No raw milk sample fulfilled the International Standards for very good quality milk for SPC or coliform count. For pasteurized milk, only 33.33% samples fulfilled the International Standards for very good pasteurized milk. SPC and coliform counts for UHT or sterilized milk was zero thus fulfilling the International Standards.
A total of 162 isolates (88 isolates from raw milk and 74 isolates from the pasteurized milk) were identified. These isolates included Staphylococcus epidermidis, 38 strain (21 from raw milk and 17 from pasteurized milk), Staph. aureus, 30 strains (16 from raw and 14 from pasteurized milk), Escherichia coli, 24 stains (14 and 10), Enterobacter aerogenes, 20 (11, 09), Bacillus cereus, 22 (15, 07), Micrococcus luteus 15, (03, 12) and Klebsiellapneumoniae 13 (08, 05) strains. vitro antibiotic sensitivity tests indicated that all the isolates were resistant to penicillin whereas most of the isolates were sensitive to Gentamicin, Kanamycin and Chloramphenicol. Escherichia coli was resistant to most of the commonly used antibiotics.
Overall hygienic quality of raw and pasteurized milk available in Lahore city was graded as poor.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0802,T (Browse shelf) | Available | 0802,T |
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The present study was conducted to study the bacteriological quality of various types of milk being marketed in Lahore. A total of 120 milk samples consisting of equal number (30 each of raw, pasteurized, UHT and sterilized milk) from various sources were examined for standard plate counts using milk agar, coliform count using EMB agar and using milk ring test (MRT) for brucellosis.
All the samples were negative for MRT. Average standard plate count (SPC) and coliform counts for raw milk were 2.34x107 cfulml and 2.2x105 cfu/ml respectively. Similarly, SPC and coliform counts for pasteurized milk were found as 2.16x105 cfuJml and 3.28x104 cfu/ml respectively. No raw milk sample fulfilled the International Standards for very good quality milk for SPC or coliform count. For pasteurized milk, only 33.33% samples fulfilled the International Standards for very good pasteurized milk. SPC and coliform counts for UHT or sterilized milk was zero thus fulfilling the International Standards.
A total of 162 isolates (88 isolates from raw milk and 74 isolates from the pasteurized milk) were identified. These isolates included Staphylococcus epidermidis, 38 strain (21 from raw milk and 17 from pasteurized milk), Staph. aureus, 30 strains (16 from raw and 14 from pasteurized milk), Escherichia coli, 24 stains (14 and 10), Enterobacter aerogenes, 20 (11, 09), Bacillus cereus, 22 (15, 07), Micrococcus luteus 15, (03, 12) and Klebsiellapneumoniae 13 (08, 05) strains. vitro antibiotic sensitivity tests indicated that all the isolates were resistant to penicillin whereas most of the isolates were sensitive to Gentamicin, Kanamycin and Chloramphenicol. Escherichia coli was resistant to most of the commonly used antibiotics.
Overall hygienic quality of raw and pasteurized milk available in Lahore city was graded as poor.
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